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Literature summary for 3.6.4.B6 extracted from

  • Banerjee, A.; Neiner, T.; Tripp, P.; Albers, S.V.
    Insights into subunit interactions in the Sulfolobus acidocaldarius archaellum cytoplasmic complex (2013), FEBS J., 280, 6141-6149.
    View publication on PubMed
Search for more literature for 3.6.4.B6

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of Strep II-tagged FlaI protein in Escherichia coli strain BL21-CodonPlus-RIL, co-expression with Strep II-tagged FlaXc and His6-tagged FlaH Sulfolobus acidocaldarius

Protein Variants

Protein Variants Comment Organism
additional information construction of various truncated versions of FlaI and interaction analysis with the archaellum subunit FlaX Sulfolobus acidocaldarius

Localization

Localization Comment Organism GeneOntology No. Textmining
archaeal-type flagellum archaellum Sulfolobus acidocaldarius 97589
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Sulfolobus acidocaldarius
-
 ADP + phosphate
-
 ?
ATP + H2O Sulfolobus acidocaldarius ATCC 33909
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4J9L0
-
-
Sulfolobus acidocaldarius ATCC 33909 Q4J9L0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Strep II-tagged FlaI, Strep II-tagged FlaXc, and His6-tagged FlaH from in Escherichia coli strain BL21-CodonPlus-RIL as ternary complex by nickel affinity chromatography, or single FlaI by avidin affinity chromatography Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Sulfolobus acidocaldarius ADP + phosphate
-
 ?
ATP + H2O
-
 Sulfolobus acidocaldarius ATCC 33909 ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer crystal structure analysis, FlaI forms hexameric species in an ATP-dependent manner Sulfolobus acidocaldarius

Synonyms

Synonyms Comment Organism
FlaI
-
 Sulfolobus acidocaldarius

General Information

General Information Comment Organism
additional information interaction analysis of fluorescent-labeled proteins FlaI, FlaX and FlaH , overview Sulfolobus acidocaldarius
physiological function the enzyme shows a dual function in the assembly and the rotation of the archaellum, the archaeal motility structure that is the functional pendant of the bacterial flagellum but is assembled by a mechanism similar to that for type IV pili. FlaX, a crenarchaeal archaellum subunit from Sulfolobus acidocaldarius, forms a ring-like oligomer, and it was proposed that this ring may act as a static platform for torque generation in archaellum rotation. FlaX acts as a cytoplasmic scaffold in archaellum assembly, as it interacts with FlaI as well as with the recA family protein FlaH, the only cytoplasmic components of the archaellum. FlaI N- and C-termini interact with FlaX. FlaI, FlaX and FlaH interact with high affinities in the nanomolar range forming the cytoplasmic motor complex of the archaellum. The FlaX ring may assemble around FlaJ, and that FlaI confers conformational change of FlaJ transfers to the membrane domain of FlaX ring, which leads to correct incorporation of archaellin (FlaB) into the newly growing archaellum filament Sulfolobus acidocaldarius