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3.6.1.65: (d)CTP diphosphatase

This is an abbreviated version!
For detailed information about (d)CTP diphosphatase, go to the full flat file.

Word Map on EC 3.6.1.65

Reaction

CTP
+
H2O
=
CMP
+
diphosphate

Synonyms

(d)CTP diphosphohydrolase, (d)CTP pyrophosphohydrolase, MazG, nudG, Orf135

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.65 (d)CTP diphosphatase

Engineering

Engineering on EC 3.6.1.65 - (d)CTP diphosphatase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D118A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
D118E
-
increase in hydrolysis of substrate 2-hydroxy-dATP
D118N
-
impaired hydrolysis of substrate 2-hydroxy-dATP
E33A
-
increase in hydrolysis of substrate 2-hydroxy-dATP
E33Q
-
increase in hydrolysis of substrate 2-hydroxy-dATP
E43A
-
substrate 5-methyl-dCTP, about 20% of wild-type activity, substrate dCTP, 1%of wild-type activity
E52A
-
complete loss of activity
E52D
-
complete loss of activity
E52Q
-
complete loss of activity
E55A
-
substrate 5-methyl-dCTP, about 20% of wild-type activity, substrate dCTP, 1%of wild-type activity
E55D
-
substrate 5-methyl-dCTP, about 40% of wild-type activity, substrate dCTP, 2% of wild-type activity
E55Q
-
substrate 5-methyl-dCTP, about 110% of wild-type activity, substrate dCTP, 10% of wild-type activity
E56A
-
complete loss of activity
E56D
-
complete loss of activity
E56Q
-
complete loss of activity
G36A
-
complete loss of activity
G37A
-
complete loss of activity
K38A
-
substrate 5-methyl-dCTP, about 30% of wild-type activity, substrate dCTP, 10% of wild-type activity
K38R
-
substrate 5-methyl-dCTP, about 40% of wild-type activity, substrate dCTP, 5% of wild-type activity
L53A
-
substrate 5-methyl-dCTP, about 30% of wild-type activity, substrate dCTP, 30% of wild-type activity
R51A
-
substrate 5-methyl-dCTP, about 5% of wild-type activity, substrate dCTP, 1% of wild-type activity
R72A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
D118A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
-
D118N
-
impaired hydrolysis of substrate 2-hydroxy-dATP
-
E33A
-
increase in hydrolysis of substrate 2-hydroxy-dATP
-
E33Q
-
increase in hydrolysis of substrate 2-hydroxy-dATP
-
R72A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
-