Information on EC 3.6.1.65 - (d)CTP diphosphatase

Word Map on EC 3.6.1.65
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
3.6.1.65
-
RECOMMENDED NAME
GeneOntology No.
(d)CTP diphosphatase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CTP + H2O = CMP + diphosphate
show the reaction diagram
(1)
-
-
-
dCTP + H2O = dCMP + diphosphate
show the reaction diagram
(2)
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pyrimidine deoxyribonucleotides dephosphorylation
-
-
pyrimidine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
(deoxy)cytidine 5'-triphosphate diphosphohydrolase
The enzyme, characterized from the bacterium Escherichia coli, is specific for the pyrimidine nucleotides CTP and dCTP. It also acts on 5-methyl-dCTP, 5-hydroxy-dCTP and 8-hydroxy-dGTP.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-hydroxy-dATP + H2O
2-hydroxy-AMP + diphosphate
show the reaction diagram
5-hydroxy-CTP + H2O
5-hydroxy-CMP + diphosphate
show the reaction diagram
-
-
-
?
5-hydroxy-dCTP + H2O
5-hydroxy-dCMP + diphosphate
show the reaction diagram
-
4.3% of the activity with 5-hydroxy-CTP
-
?
5-methyl-dCTP + H2O
5-methyl-dCMP + diphosphate
show the reaction diagram
8-hydroxy-dGTP + H2O
8-hydroxy-dGMP + diphosphate
show the reaction diagram
arabinosyl-CTP + H2O
arabinosyl-CMP + diphosphate
show the reaction diagram
-
3% of the activity with 5-methyl-dCTP
-
-
?
CDP + H2O
cytidine + diphosphate
show the reaction diagram
-
2% of the activity with 5-methyl-dCTP
-
-
?
CTP + H2O
CMP + diphosphate
show the reaction diagram
dCTP + H2O
dCMP + diphosphate
show the reaction diagram
dTTP + H2O
dTMP + diphosphate
show the reaction diagram
-
2% of the activity with 5-methyl-dCTP
-
-
?
dUTP + H2O
dUMP + diphosphate
show the reaction diagram
-
1% of the activity with 5-methyl-dCTP
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
about 10% of the activity with Mg2+
additional information
-
absolute requirement for a divalnt cation. No activity in presence of Zn2+, Co2+, Ca2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0055 - 0.31
2-hydroxy-dATP
0.0557
5-hydroxy-CTP
-
pH 8.5, 30C
0.0057 - 0.24
5-Methyl-dCTP
0.41
8-hydroxy-dGTP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.428 - 0.551
CTP
0.65 - 1.72
dCTP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17 - 2.7
2-hydroxy-dATP
35.1
5-hydroxy-CTP
Escherichia coli
-
pH 8.5, 30C
2.8 - 51.7
5-Methyl-dCTP
0.89
8-hydroxy-dGTP
Escherichia coli
-
wild-type, pH not specified in the publication, temperature not specified in the publication
9.28 - 25.8
CTP
2.1 - 17.4
dCTP
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.6 - 82
2-hydroxy-dATP
2804
630
5-hydroxy-CTP
Escherichia coli
-
pH 8.5, 30C
42488
13 - 1400
5-Methyl-dCTP
3059
2.2
8-hydroxy-dGTP
Escherichia coli
-
wild-type, pH not specified in the publication, temperature not specified in the publication
42487
21.7 - 47
CTP
60
2.3 - 18
dCTP
179
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
193
-
substrate 5-methyl-dCTP, pH 9.0, 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 9
-
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as glutathione S-transferase fusion protein
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D118A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
D118E
-
increase in hydrolysis of substrate 2-hydroxy-dATP
D118N
-
impaired hydrolysis of substrate 2-hydroxy-dATP
E33A
-
increase in hydrolysis of substrate 2-hydroxy-dATP
E33Q
-
increase in hydrolysis of substrate 2-hydroxy-dATP
E43A
-
substrate 5-methyl-dCTP, about 20% of wild-type activity, substrate dCTP, 1%of wild-type activity
E52A
-
complete loss of activity
E52D
-
complete loss of activity
E52Q
-
complete loss of activity
E55A
-
substrate 5-methyl-dCTP, about 20% of wild-type activity, substrate dCTP, 1%of wild-type activity
E55D
-
substrate 5-methyl-dCTP, about 40% of wild-type activity, substrate dCTP, 2% of wild-type activity
E55Q
-
substrate 5-methyl-dCTP, about 110% of wild-type activity, substrate dCTP, 10% of wild-type activity
E56A
-
complete loss of activity
E56D
-
complete loss of activity
E56Q
-
complete loss of activity
G36A
-
complete loss of activity
G37A
-
complete loss of activity
K38A
-
substrate 5-methyl-dCTP, about 30% of wild-type activity, substrate dCTP, 10% of wild-type activity
K38R
-
substrate 5-methyl-dCTP, about 40% of wild-type activity, substrate dCTP, 5% of wild-type activity
L53A
-
substrate 5-methyl-dCTP, about 30% of wild-type activity, substrate dCTP, 30% of wild-type activity
R51A
-
substrate 5-methyl-dCTP, about 5% of wild-type activity, substrate dCTP, 1% of wild-type activity
R72A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
D118A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
-
D118N
-
impaired hydrolysis of substrate 2-hydroxy-dATP
-
E33A
-
increase in hydrolysis of substrate 2-hydroxy-dATP
-
E33Q
-
increase in hydrolysis of substrate 2-hydroxy-dATP
-
R72A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
-
Show AA Sequence (138 entries)
Please use the Sequence Search for a specific query.