Protein Variants | Comment | Organism |
---|---|---|
D118A | impaired hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
D118E | increase in hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
D118N | impaired hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
E33A | increase in hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
E33Q | increase in hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
R72A | impaired hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
2-hydroxy-dATP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.0057 | - |
5-Methyl-dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.0069 | - |
5-Methyl-dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.015 | - |
5-Methyl-dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.027 | - |
2-hydroxy-dATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.028 | - |
5-Methyl-dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.067 | - |
2-hydroxy-dATP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.19 | - |
2-hydroxy-dATP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.22 | - |
5-Methyl-dCTP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.24 | - |
5-Methyl-dCTP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.31 | - |
2-hydroxy-dATP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.41 | - |
8-hydroxy-dGTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.65 | - |
dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.77 | - |
dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.93 | - |
dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.99 | - |
dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli JD22899 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-dATP + H2O | - |
Escherichia coli | 2-hydroxy-dAMP + diphosphate | - |
? | |
2-hydroxy-dATP + H2O | - |
Escherichia coli JD22899 | 2-hydroxy-dAMP + diphosphate | - |
? | |
5-methyl-dCTP + H2O | - |
Escherichia coli | 5-methyl-dCMP + diphosphate | - |
? | |
5-methyl-dCTP + H2O | - |
Escherichia coli JD22899 | 5-methyl-dCMP + diphosphate | - |
? | |
8-hydroxy-dGTP + H2O | - |
Escherichia coli | 8-hydroxy-dGMP + diphosphate | - |
? | |
8-hydroxy-dGTP + H2O | - |
Escherichia coli JD22899 | 8-hydroxy-dGMP + diphosphate | - |
? | |
dCTP + H2O | - |
Escherichia coli | dCMP + diphosphate | - |
? | |
dCTP + H2O | - |
Escherichia coli JD22899 | dCMP + diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
2-hydroxy-dATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.45 | - |
2-hydroxy-dATP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.89 | - |
8-hydroxy-dGTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1.4 | - |
2-hydroxy-dATP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1.42 | - |
2-hydroxy-dATP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2.1 | - |
dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2.7 | - |
2-hydroxy-dATP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2.7 | - |
dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2.8 | - |
5-Methyl-dCTP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
6.3 | - |
dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
6.5 | - |
5-Methyl-dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
7.7 | - |
5-Methyl-dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
8.8 | - |
dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
9.1 | - |
5-Methyl-dCTP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
11 | - |
5-Methyl-dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
35 | - |
5-Methyl-dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
8-hydroxy-dGTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2.3 | - |
dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
3.5 | - |
dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6 | - |
2-hydroxy-dATP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
6.4 | - |
dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
6.4 | - |
2-hydroxy-dATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
13 | - |
5-Methyl-dCTP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
14 | - |
2-hydroxy-dATP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
14 | - |
dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
21 | - |
2-hydroxy-dATP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
38 | - |
5-Methyl-dCTP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
82 | - |
2-hydroxy-dATP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
780 | - |
5-Methyl-dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
944 | - |
5-Methyl-dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1300 | - |
5-Methyl-dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1400 | - |
5-Methyl-dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |