Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.6.1.39: thymidine-triphosphatase

This is an abbreviated version!
For detailed information about thymidine-triphosphatase, go to the full flat file.

Word Map on EC 3.6.1.39

Reaction

dTTP
+
H2O
=
dTDP
 +
phosphate

Synonyms

desoxythymidine-5'-triphosphatase, dTTPase, dTTPase-dUTPase, gene 4 protein, Gp4, T7 DNA helicase, T7 helicase, thymidine triphosphate nucleotidohydrolase

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.39 thymidine-triphosphatase

Engineering

Engineering on EC 3.6.1.39 - thymidine-triphosphatase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E343D
-
the mutant does not hydrolyze dTTP
E343N
-
the mutant does not hydrolyze dTTP
E343Q
N468A
-
the mutant hydrolyzes dTTP at rates comparable with wild type enzyme. N468A hydrolyzes nearly 4fold less efficiently than the wild type enzyme in the absence of single-stranded DNA and is not stimulated by the presence of single-stranded DNA
R493A/N468A
-
the mutant hydrolyzes dTTP at rates comparable with wild type enzyme and is not stimulated by the presence of single-stranded DNA
R493Q