3.6.1.39: thymidine-triphosphatase
This is an abbreviated version!
For detailed information about thymidine-triphosphatase, go to the full flat file.
Word Map on EC 3.6.1.39
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3.6.1.39
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bacteriophage
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single-stranded
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strand
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primase
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unwind
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duplex
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fork
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hexamer
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oligoribonucleotides
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dna-dependent
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ring-shaped
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richardson
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helicase-primase
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primase-helicase
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tetraribonucleotides
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replisome
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template-directed
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okazaki
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rna-primed
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dnab
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medicine
- 3.6.1.39
- bacteriophage
-
single-stranded
- strand
- primase
-
unwind
- duplex
- fork
-
hexamer
- oligoribonucleotides
-
dna-dependent
-
ring-shaped
-
richardson
-
helicase-primase
-
primase-helicase
-
tetraribonucleotides
-
replisome
-
template-directed
-
okazaki
-
rna-primed
- dnab
- medicine
Reaction
Synonyms
desoxythymidine-5'-triphosphatase, dTTPase, dTTPase-dUTPase, gene 4 protein, Gp4, T7 DNA helicase, T7 helicase, thymidine triphosphate nucleotidohydrolase
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Substrates Products
Substrates Products on EC 3.6.1.39 - thymidine-triphosphatase
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REACTION DIAGRAM
additional information
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wild type gp4 helicase unwinds the duplex DNA
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dCTP + H2O
dCDP + phosphate
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60% activity compared with dTTP as substrate
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?
dTTP + H2O
dTDP + phosphate
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mechanisms of DNA-dependent and-independent dTTP hydrolysis by the gene 4 protein of bacteriophage T7 differ in the pathways by which these reactions are catalyzed. In the presence of dTTP, gene 4 protein monomers assemble as a ring that binds single-stranded DNA and couples the hydrolysis of dTTP to unidirectional translocation and the unwinding of duplex DNA, overview
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?
dTTP + H2O
dTDP + phosphate
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the lysines on loop II are essential for ssDNA binding
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-
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dTTP + H2O
dTDP + phosphate
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dTTP hydrolysis is coupled to DNA unwinding, dependence of dTTP hydrolysis rates on dsDNA stability, the force-producing step during DNA unwinding is not associated with dTTP binding, but dTTP hydrolysis or phosphate release
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?
dTTP + H2O
dTDP + phosphate
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dTTP as part of DNA molecules, activity assay using four different DNA substrates with the same GC content of 5%, but varying lengths of duplex regions, fluorescent substrates, mechanochemistry and kinetics of the DNA-unwinding reaction, overview
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-
?
dTTP + H2O
dTDP + phosphate
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Gp4 binds, as a hexamer, to single-stranded DNA in the presence of dTTP and translocates in a 5' to 3' direction along the DNA strand using the energy derived from the hydrolysis of dTTP
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dUTP + H2O
dUDP + phosphate
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50% activity compared with dTTP as substrate
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?
dUTP + H2O
dUDP + phosphate
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50% activity compared with dTTP as substrate
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?
dUTP + H2O
dUMP + diphosphate
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70% activity compared with dTTP as substrate
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?
UDP + phosphate
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20% activity compared with dTTP as substrate
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?
UTP + H2O
UDP + phosphate
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20% activity compared with dTTP as substrate
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?