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Literature summary for 3.6.1.39 extracted from

  • Crampton, D.J.; Mukherjee, S.; Richardson, C.C.
    DNA-induced switch from independent to sequential dTTP hydrolysis in the bacteriophage T7 DNA helicase (2006), Mol. Cell, 21, 165-174.
    View publication on PubMed
Search for more literature for 3.6.1.39

Protein Variants

Protein Variants Comment Organism
E343Q site-directed mutagenesis, the mutant is inactive, dTTP hydrolysis and DNA unwinding activities are abolished, but the mutant shows increased single-stranded DNA binding affinity compared to the wild-type enzyme, overview Escherichia phage T7

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia phage T7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTTP + H2O Escherichia phage T7 mechanisms of DNA-dependent and-independent dTTP hydrolysis by the gene 4 protein of bacteriophage T7 differ in the pathways by which these reactions are catalyzed. In the presence of dTTP, gene 4 protein monomers assemble as a ring that binds single-stranded DNA and couples the hydrolysis of dTTP to unidirectional translocation and the unwinding of duplex DNA, overview dTDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia phage T7
-
 the enzyme is encoded on gene 4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + H2O mechanisms of DNA-dependent and-independent dTTP hydrolysis by the gene 4 protein of bacteriophage T7 differ in the pathways by which these reactions are catalyzed. In the presence of dTTP, gene 4 protein monomers assemble as a ring that binds single-stranded DNA and couples the hydrolysis of dTTP to unidirectional translocation and the unwinding of duplex DNA, overview Escherichia phage T7 dTDP + phosphate
-
 ?
dTTP + H2O the lysines on loop II are essential for ssDNA binding Escherichia phage T7 dTDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
More all subunits are catalytic Escherichia phage T7

Synonyms

Synonyms Comment Organism
dTTPase
-
 Escherichia phage T7

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 DNA binding and dTTPase assays at Escherichia phage T7

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 DNA binding and dTTPase assays at Escherichia phage T7