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3.5.99.7: 1-aminocyclopropane-1-carboxylate deaminase

This is an abbreviated version!
For detailed information about 1-aminocyclopropane-1-carboxylate deaminase, go to the full flat file.

Word Map on EC 3.5.99.7

Reaction

1-aminocyclopropane-1-carboxylate
+
H2O
=
2-oxobutanoate
 +
NH3

Synonyms

1-aminocyclopropane-1-carboxylate deaminase, 1-aminocyclopropane-1-carboxylate endolyase (deaminating), 1-aminocyclopropane-1-carboxylic acid deaminase, 1-aminocyclopropane-1-carboxylic acid-deaminase, ACC deaminase, ACC-deaminase, ACCD, ACD, AcdS, ACPC deaminase, AMETH_2787, aspB, BC_3216, DA46_1949, deaminase, 1-aminocyclopropane-1-carboxylate, EC 4.1.99.4, More, PST_1815

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.99 In other compounds
                3.5.99.7 1-aminocyclopropane-1-carboxylate deaminase

Cloned

Cloned on EC 3.5.99.7 - 1-aminocyclopropane-1-carboxylate deaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ACC-deaminase encoding gene acdS, and a regulator gene lrp, i.e. acdR
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An ACC deaminase minus mutant (AcdS) is constructed by the insertion of a tetracycline resistance gene into the coding region of the bacterial ACC deaminase gene.
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cloning and genotype of Pseudomonas brassicacearum strains, overview
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DNA and amino acid sequence determination and analysis, phylogenetic analysis
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DNA and amino acid sequence determination and analysis, sequence compariosn and phylogenetic analysis
enzyme expression in Agrobacterium tumefaciens strain C58 leading to increased gene transfer of the recombinant bacterium in transfected plant cells, overview
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expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli Rosetta(DE3) cells
expressed in Escherichia coli strain DH5alpha
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expressed in Escherichia coli strain DH5alphaPRO
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expression in Escherichia coli
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expression in Escherichia coli and in delayed ripening tomato
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gene acdS, DNA and amino acid sequence determination and analysis, gene mapping and phylogenetic analysis, fate of the acdS/acdR locus during phenotypic variation in Azospirillum lipoferum 4B
gene acdS, DNA and amino acid sequence determination and analysis, genotyping
gene acdS, DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis
gene acdS, DNA and amino acid sequence determination and analysis, genotyping, phylogenetic analysis and tree
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gene acdS, DNA and amino acid sequence determination, phylogenetic analysis using 16S RNA genetic sequence
gene acdS, functional recombinant expression of the exogenous 1-aminocyclopropane-1-carboxylate deaminase gene from Pseudomonas putida in the psychrotolerant bacteria, acdS negative Flavobacterium sp. strain OR306 and Pseudomonas frederiksbergensis strain OS211
gene acdS, phylogenetic analysis
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gene acdS, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta
gene acdS, recombinant expression of His6-tagged ACCD in Escherichia coli strain BL21(DE3)
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gene acdS, recombinant overexpression in Sinorhizobium meliloti strain CCNWSX0020 (ACCC 19736), a Cu-resistant strain isolated from the root nodules of Medicago lupulina plants growing in lead-zinc mine tailings in China, quantitative real-time PCR analysis
gene acdS, RT-PCR enzyme expression analysis
gene acdS, semi-quantitative RT-PCR expression analysis
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gene acdS, sequence comparisons and phylogenetic analysis
gene PST_1815 or acdS, DNA and amino acid sequence determination and analysis, sequence comparisons, quntitative RT-PCR expression analysis, transcriptional organization of the three consecutive genes, PST1814-16, in the genome of Pseudomonas stutzeri strain A1501, overview
identification of a specific ACC deaminase domain region (ACCD-DR) that, when PCR amplified from the soil, produces a variant pool that can be swapped into functional plasmids carrying ACC deaminase-encoding genes. The ACC deaminase domain regions are recombinantly expressed in Escherichia coli. Functional clones of ACC deaminase are selected for in a competition assay based on their capacity to provide nitrogen to Escherichia coli in vitro, DNA and amino acid sequence determination and analysis
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Pseudomonas putida UW4 ACC deaminase is cloned into the pET30a (+) vector at the EcoRV/HindIII sites. All single and double mutants are constructed using a Phusion Site Directed Mutagenesis Kit.
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subcloned into pET-11d and expressed in Escherichia coli
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Using non-transformed canola (Brassica napus) or canola transformed with the ACC deaminase gene from Pseudomonas putida UW4. The transformed canola line has two copies of the ACC deaminase gene under the control of the rootspecific Agrobacterium rhizogenes promoter. This homozygous line is created through Agrobacterium tumefaciens transformation of canola callus culture.
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wild-type enzyme and mutants Y268F and Y294F as His6-tagged proteins in Escherichia coli
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