Cloned (Comment) | Organism |
---|---|
gene acdS, DNA and amino acid sequence determination, phylogenetic analysis using 16S RNA genetic sequence | Enterobacter cloacae |
gene acdS, DNA and amino acid sequence determination, phylogenetic analysis using 16S RNA genetic sequence | Enterobacter cancerogenus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.08 | - |
1-aminocyclopropane-1-carboxylate | pH 7.5, 30°C | Enterobacter cloacae | |
12.5 | - |
1-aminocyclopropane-1-carboxylate | pH 7.5, 30°C | Enterobacter cancerogenus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K2HPO4 | results in highest activity | Enterobacter cloacae | |
MgSO4 | results in highest activity | Enterobacter cancerogenus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + H2O | Enterobacter cloacae | - |
2-oxobutanoate + NH3 | - |
? | |
1-aminocyclopropane-1-carboxylate + H2O | Enterobacter cancerogenus | - |
2-oxobutanoate + NH3 | - |
? | |
1-aminocyclopropane-1-carboxylate + H2O | Enterobacter cancerogenus MSA2 | - |
2-oxobutanoate + NH3 | - |
? | |
1-aminocyclopropane-1-carboxylate + H2O | Enterobacter cloacae MSA1 | - |
2-oxobutanoate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cancerogenus | - |
recovered from the rhizosphere of Jatropha sp., gene acdS | - |
Enterobacter cancerogenus MSA2 | - |
recovered from the rhizosphere of Jatropha sp., gene acdS | - |
Enterobacter cloacae | - |
recovered from the rhizosphere of Jatropha sp., gene acdS | - |
Enterobacter cloacae MSA1 | - |
recovered from the rhizosphere of Jatropha sp., gene acdS | - |
Purification (Comment) | Organism |
---|---|
native enzyme by ammonium sulfate fractionation, and anion exchange chromatography | Enterobacter cloacae |
native enzyme by ammonium sulfate fractionation, and anion exchange chromatography | Enterobacter cancerogenus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + H2O | - |
Enterobacter cloacae | 2-oxobutanoate + NH3 | - |
? | |
1-aminocyclopropane-1-carboxylate + H2O | - |
Enterobacter cancerogenus | 2-oxobutanoate + NH3 | - |
? | |
1-aminocyclopropane-1-carboxylate + H2O | - |
Enterobacter cancerogenus MSA2 | 2-oxobutanoate + NH3 | - |
? | |
1-aminocyclopropane-1-carboxylate + H2O | - |
Enterobacter cloacae MSA1 | 2-oxobutanoate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 35000-42000, SDS-PAGE | Enterobacter cloacae |
multimer | x * 35000-42000, SDS-PAGE | Enterobacter cancerogenus |
Synonyms | Comment | Organism |
---|---|---|
ACC deaminase | - |
Enterobacter cloacae |
ACC deaminase | - |
Enterobacter cancerogenus |
ACC-deaminase | - |
Enterobacter cloacae |
ACC-deaminase | - |
Enterobacter cancerogenus |
More | formerly EC 4.1.99.4 | Enterobacter cloacae |
More | formerly EC 4.1.99.4 | Enterobacter cancerogenus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
- |
Enterobacter cloacae |
28 | - |
- |
Enterobacter cancerogenus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | activity range, optimum activity at 28°C, rapid decrease in activity above 30°C, no activity at 60°C | Enterobacter cloacae |
25 | 50 | activity range, optimum activity at 28°C, rapid decrease in activity above 30°C, no activity at 60°C | Enterobacter cancerogenus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Enterobacter cloacae |
7.5 | - |
- |
Enterobacter cancerogenus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | tightly bound to the enzyme in the amount of approximately one molecule per subunit | Enterobacter cloacae | |
pyridoxal 5'-phosphate | tightly bound to the enzyme in the amount of approximately one molecule per subunit | Enterobacter cancerogenus |