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Literature summary for 3.5.99.7 extracted from

  • Fedorov, D.N.; Ekimova, G.A.; Doronina, N.V.; Trotsenko, Y.A.
    1-Aminocyclopropane-1-carboxylate (ACC) deaminases from Methylobacterium radiotolerans and Methylobacterium nodulans with higher specificity for ACC (2013), FEMS Microbiol. Lett., 343, 70-76.
    View publication on PubMed
Search for more literature for 3.5.99.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Methylobacterium nodulans
expressed in Escherichia coli Rosetta(DE3) cells Methylobacterium radiotolerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 1-aminocyclopropane-1-carboxylate at pH 8.0 and 30°C Methylobacterium nodulans
1.8
-
 1-aminocyclopropane-1-carboxylate at pH 8.0 and 30°C Methylobacterium radiotolerans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 4 * 36000, SDS-PAGE Methylobacterium nodulans
36000
-
 4 * 36000, SDS-PAGE Methylobacterium radiotolerans
144000
-
 gel filtration Methylobacterium nodulans
144000
-
 gel filtration Methylobacterium radiotolerans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-aminocyclopropane-1-carboxylate + H2O Methylobacterium nodulans
-
 2-oxobutanoate + NH3
-
 ?
1-aminocyclopropane-1-carboxylate + H2O Methylobacterium radiotolerans
-
 2-oxobutanoate + NH3
-
 ?
1-aminocyclopropane-1-carboxylate + H2O Methylobacterium radiotolerans JCM 2831
-
 2-oxobutanoate + NH3
-
 ?
1-aminocyclopropane-1-carboxylate + H2O Methylobacterium nodulans ORS 2060
-
 2-oxobutanoate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Methylobacterium nodulans B8IP05
-
-
Methylobacterium nodulans ORS 2060 B8IP05
-
-
Methylobacterium radiotolerans B1M5C5
-
-
Methylobacterium radiotolerans JCM 2831 B1M5C5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Methylobacterium nodulans
Ni-NTA agarose column chromatography Methylobacterium radiotolerans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.44
-
 purified enzyme, at pH 8.0 and 30°C Methylobacterium radiotolerans
0.74
-
 purified enzyme, at pH 8.0 and 30°C Methylobacterium nodulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-aminocyclopropane-1-carboxylate + H2O
-
 Methylobacterium nodulans 2-oxobutanoate + NH3
-
 ?
1-aminocyclopropane-1-carboxylate + H2O
-
 Methylobacterium radiotolerans 2-oxobutanoate + NH3
-
 ?
1-aminocyclopropane-1-carboxylate + H2O
-
 Methylobacterium radiotolerans JCM 2831 2-oxobutanoate + NH3
-
 ?
1-aminocyclopropane-1-carboxylate + H2O
-
 Methylobacterium nodulans ORS 2060 2-oxobutanoate + NH3
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 36000, SDS-PAGE Methylobacterium nodulans
homotetramer 4 * 36000, SDS-PAGE Methylobacterium radiotolerans

Synonyms

Synonyms Comment Organism
ACC deaminase
-
 Methylobacterium nodulans
ACC deaminase
-
 Methylobacterium radiotolerans
AcdS
-
 Methylobacterium nodulans
AcdS
-
 Methylobacterium radiotolerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Methylobacterium radiotolerans
50
-
-
 Methylobacterium nodulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 1-aminocyclopropane-1-carboxylate at pH 8.0 and 30°C Methylobacterium radiotolerans
1.86
-
 1-aminocyclopropane-1-carboxylate at pH 8.0 and 30°C Methylobacterium nodulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Methylobacterium nodulans
8
-
-
 Methylobacterium radiotolerans

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Methylobacterium nodulans
pyridoxal 5'-phosphate
-
 Methylobacterium radiotolerans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.61
-
 1-aminocyclopropane-1-carboxylate at pH 8.0 and 30°C Methylobacterium radiotolerans
2.33
-
 1-aminocyclopropane-1-carboxylate at pH 8.0 and 30°C Methylobacterium nodulans