3.5.2.20: isatin hydrolase

This is an abbreviated version!
For detailed information about isatin hydrolase, go to the full flat file.

Reaction

Synonyms

IH-b, isatin amidohydrolase, isatin hydrolase A, isatin hydrolase b, LaIHA, RsIHA

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.2 In cyclic amides

3.5.2.20 isatin hydrolase

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Results	in table
26	AA Sequence
2	Application
4	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
8	General Information
1	Inhibitors
1	Ki Value [mM]
3	KM Value [mM]
6	Metals/Ions
11	Natural Substrates/ Products (Substrates)
18	Organism
1	Pathway
4	pH Optimum
1	pH Range
2	Protein Variants
4	Purification (Commentary)
1	Reaction
16	Reference
5	Specific Activity [micromol/min/mg]
21	Substrates and Products (Substrate)
19	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
3	Temperature Stability [°C]
2	Turnover Number [1/s]

Engineering

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Engineering on EC 3.5.2.20 - isatin hydrolase

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S225A

Roseibium aggregatum

A0NLY7

no significant difference in Km or kcat/Km is observed between wild-type enzyme and mutant enzyme. Decrease in melting temperature of 2°C, as compared to wild-type enzyme

730050

S225C

Roseibium aggregatum

A0NLY7

the S225C mutant is catalytically activated by the mutation. the biophysical properties of the of the wt and S225C enzyme appear unchanged

730050