Information on EC 3.5.2.20 - isatin hydrolase

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The expected taxonomic range for this enzyme is: Alphaproteobacteria

EC NUMBER
COMMENTARY hide
3.5.2.20
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RECOMMENDED NAME
GeneOntology No.
isatin hydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
isatin + H2O = isatinate
show the reaction diagram
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
indole-3-acetate degradation
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SYSTEMATIC NAME
IUBMB Comments
isatin amidohydrolase
Requires Mn2+. This enzyme, found in several bacterial species, is involved in the degradation of indole-3-acetic acid.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
isatin + H2O
isatinate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
isatin + H2O
isatinate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thioisatinate
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011
isatin
-
pH 9.0, 32°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
thioisatinate
pH 8.0, temperature not specified in the publication
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.34
-
pH 9.0, 32°C
0.41
-
pH 9.0, 32°C
0.611
-
pH 9.0, 32°C
0.64
-
pH 9.0, 32°C
0.69
-
pH 9.0, 32°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structures of isatin hydrolase from Labrenzia aggregata in the apo and the product state. Apo-isatin hydrolase isoform b is crystallized by vapor diffusion using sitting drop trays with a protein concentration of 20 mg/ml. Crystals appear in 0.2 M calcium acetate, 16% (w/v) PEG3350, and 1 mM MnCl2 and grew within 48 h
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51
melting temperature of mutant enzyme S225A
53
melting te,perature of wild-type enzyme and mutant enzyme S225C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the His6-containing isatin hydrolase isoform b is expressed in Eschertichia coli BL21 AI cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is inducible (4-5-fold) by its substrate, isatin
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S225A
no significant difference in Km or kcat/Km is observed between wild-type enzyme and mutant enzyme. Decrease in melting temperature of 2°C, as compared to wild-type enzyme
S225C
the S225C mutant is catalytically activated by the mutation. the biophysical properties of the of the wt and S225C enzyme appear unchanged