3.5.2.20: isatin hydrolase

This is an abbreviated version!
For detailed information about isatin hydrolase, go to the full flat file.

Reaction

Synonyms

IH-b, isatin amidohydrolase, isatin hydrolase A, isatin hydrolase b, LaIHA, RsIHA

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.2 In cyclic amides

3.5.2.20 isatin hydrolase

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Results	in table
26	AA Sequence
2	Application
4	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
8	General Information
1	Inhibitors
1	Ki Value [mM]
3	KM Value [mM]
6	Metals/Ions
11	Natural Substrates/ Products (Substrates)
18	Organism
1	Pathway
4	pH Optimum
1	pH Range
2	Protein Variants
4	Purification (Commentary)
1	Reaction
16	Reference
5	Specific Activity [micromol/min/mg]
21	Substrates and Products (Substrate)
19	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
3	Temperature Stability [°C]
2	Turnover Number [1/s]

Crystallization

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Crystallization on EC 3.5.2.20 - isatin hydrolase

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crystallized at 22°C using a protein concentration of 15 mg/ml in 5 mM Bis-Tris pH 7.0, 100 mM NaCl, and 1.0 mM MnCl2. The enzyme is crystallised by vapour diffusion using sitting drops and by vapour diffusion using hanging drops

Ralstonia solanacearum

Q8XYC3

755492

crystal structures of isatin hydrolase from Labrenzia aggregata in the apo and the product state. Apo-isatin hydrolase isoform b is crystallized by vapor diffusion using sitting drop trays with a protein concentration of 20 mg/ml. Crystals appear in 0.2 M calcium acetate, 16% (w/v) PEG3350, and 1 mM MnCl2 and grew within 48 h

Roseibium aggregatum

Roseibium aggregatum