3.5.1.46: 6-aminohexanoate-oligomer exohydrolase
This is an abbreviated version!
For detailed information about 6-aminohexanoate-oligomer exohydrolase, go to the full flat file.
Word Map on EC 3.5.1.46
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3.5.1.46
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flavobacterium
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arthrobacter
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nylon-6
-
amide
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beta-lactamase
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carboxylesterase
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esterase
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esterolytic
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hydrolases
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vapour-diffusion
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tetrahedral
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penicillin-recognizing
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man-made
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sitting-drop
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compendium
- 3.5.1.46
- flavobacterium
- arthrobacter
- nylon-6
- amide
- beta-lactamase
- carboxylesterase
- esterase
-
esterolytic
- hydrolases
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vapour-diffusion
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tetrahedral
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penicillin-recognizing
-
man-made
-
sitting-drop
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compendium
Reaction
Synonyms
6-aminohexanoate dimer hydrolase, 6-aminohexanoate oligomer hydrolase, 6-aminohexanoate-dimer hydrolase, 6-aminohexanoic acid oligomer hydrolase, Ahx dimer hydrolase, EII, EII', Hyb-24, Hyb-24DN, N-(6-aminohexanoyl)-6-aminohexanoate amidohydrolase, NylB, NylCA, NylCK, NylCP2, nylon oligomer hydrolase, Nylon oligomers degrading enzyme EII, Nylon oligomers degrading enzyme EII', nylon-oligomer degrading enzyme
ECTree
3.5.1.46 6-aminohexanoate-oligomer exohydrolaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.5.1.46 - 6-aminohexanoate-oligomer exohydrolase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N-(8-aminooctanoyl)-6-aminohexanoic acid + H2O
8-aminooctanoic acid + 6-aminohexanoic acid
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low activity for enzyme EII, EII' no activity
-
?
6-aminohexanoate dimer + H2O
2 6-aminohexanoate
-
-
-
?
6-aminohexanoate dimer + H2O
2 6-aminohexanoate
the enzyme hydrolyzes the Ahx oligomers by an exo-type mechanism
-
-
?
N-(4-aminobutyryl)-4-aminobutyric acid + H2O
4-aminobutyric acid + 4-aminobutyric acid
-
very low activity for EII
-
?
N-(4-aminobutyryl)-4-aminobutyric acid + H2O
4-aminobutyric acid + 4-aminobutyric acid
-
very low activity for EII
-
?
N-(4-nitrophenyl)-6-aminohexanamide + H2O
4-nitroaniline + 6-aminohexanoate
-
-
-
-
?
N-(4-nitrophenyl)-6-aminohexanamide + H2O
4-nitroaniline + 6-aminohexanoate
-
-
-
-
?
N-(4-nitrophenyl)-6-aminohexanamide + H2O
4-nitroaniline + 6-aminohexanoate
-
-
-
-
?
N-(4-nitrophenyl)-6-aminohexanamide + H2O
4-nitroaniline + 6-aminohexanoate
-
-
-
-
?
N-(6-aminohexanoyl)-4-aminobutyric acid + H2O
6-aminohexanoic acid + 4-aminobutyric acid
-
low activity for EII
-
?
N-(6-aminohexanoyl)-4-aminobutyric acid + H2O
6-aminohexanoic acid + 4-aminobutyric acid
-
low activity for EII
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
2 6-aminohexanoate
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the enzyme is involved degradation of nylon-6
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
2 6-aminohexanoate
-
-
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
2 6-aminohexanoate
-
the enzyme is involved degradation of nylon-6
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
2 6-aminohexanoate
-
the enzyme is involved degradation of nylon-6
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
2 6-aminohexanoate
-
the enzyme is involved degradation of nylon-6
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate
-
the enzyme is responsible for the degradation of nylon-6 industrial production by-products
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate
-
isozyme EII' shows only about 0.5% of the activity of EII
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate
-
the enzyme hydrolyzes the linear dimer substrate
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
mutant protein D181N 20fold less active, mutant D181E 200fold less active
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
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specific acitvity for EII' 0.5% that of EII
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
specific acitvity for EII' 0.5% that of EII
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
mutant protein D181N 20fold less active, mutant D181E 200fold less active
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
-
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
6-aminohexanoate + 6-aminohexanoate
-
-
-
?
N-(6-aminohexanoyl)-8-aminooctanoic acid + H2O
6-aminohexanoate + 8-aminooctanoate
-
4fold higher activity than for N-(6-aminohexanoyl)-6-aminohexanoate for enzyme EII, EII' poorly active
-
?
N-(6-aminohexanoyl)-8-aminooctanoic acid + H2O
6-aminohexanoate + 8-aminooctanoate
-
4fold higher activity than for N-(6-aminohexanoyl)-6-aminohexanoate for enzyme EII, EII' poorly active
-
?
N-(6-aminohexanoyl)-aniline + H2O
6-aminohexanoate + aniline
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highest activity for enzyme EII, EII' low activity
-
?
N-(6-aminohexanoyl)-aniline + H2O
6-aminohexanoate + aniline
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highest activity for enzyme EII, EII' low activity
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?
N-6-aminohexanoate hexamer + H2O
6-aminohexanoate + ?
-
-
-
?
N-6-aminohexanoate icosamer + H2O
6-aminohexanoate + ?
-
poor activity, decreasing hydrolysis with increasing polymerization number of oligomers, higher oligomers not hydrolyzed
-
?
N-6-aminohexanoate icosamer + H2O
6-aminohexanoate + ?
-
poor activity, decreasing hydrolysis with increasing polymerization number of oligomers, higher oligomers not hydrolyzed
-
?
N-6-aminohexanoate pentamer + H2O
6-aminohexanoate + ?
-
-
-
?
N-6-aminohexanoate pentamer + H2O
6-aminohexanoate + ?
-
-
-
?
N-6-aminohexanoate pentamer + H2O
6-aminohexanoate + ?
-
-
-
?
N-6-aminohexanoate pentamer + H2O
6-aminohexanoate + ?
-
-
-
?
N-6-aminohexanoate tetramer + H2O
6-aminohexanoate + N-6-aminohexanoate trimer
-
-
-
?
N-6-aminohexanoate tetramer + H2O
6-aminohexanoate + N-6-aminohexanoate trimer
-
-
-
?
N-6-aminohexanoate tetramer + H2O
6-aminohexanoate + N-6-aminohexanoate trimer
-
-
-
?
N-6-aminohexanoate tetramer + H2O
6-aminohexanoate + N-6-aminohexanoate trimer
-
-
-
?
N-6-aminohexanoate tetramer + H2O
6-aminohexanoate + N-6-aminohexanoate trimer
-
-
-
?
N-6-aminohexanoate tetramer + H2O
6-aminohexanoate + N-6-aminohexanoate trimer
-
-
-
?
N-6-aminohexanoate tetramer + H2O
6-aminohexanoate + N-6-aminohexanoate trimer
-
-
-
?
N-6-aminohexanoate tetramer + H2O
6-aminohexanoate + N-6-aminohexanoate trimer
-
highest relative activity
major products, the trimer is subsequently subjected to hydrolysis, digests exogenously linear oligomers from the N-terminus as monomeric units
?
N-6-aminohexanoate tetramer + H2O
6-aminohexanoate + N-6-aminohexanoate trimer
-
highest relative activity
major products, the trimer is subsequently subjected to hydrolysis, digests exogenously linear oligomers from the N-terminus as monomeric units
?
N-6-aminohexanoate trimer + H2O
6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
-
-
?
N-6-aminohexanoate trimer + H2O
6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
-
-
?
N-6-aminohexanoate trimer + H2O
6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
-
-
?
N-6-aminohexanoate trimer + H2O
6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
-
-
?
N-6-aminohexanoate trimer + H2O
6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
-
-
?
N-6-aminohexanoate trimer + H2O
6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
-
-
?
N-6-aminohexanoate trimer + H2O
6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
-
-
?
N-6-aminohexanoate trimer + H2O
6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
-
-
?
N-6-aminohexanoate trimer + H2O
6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
-
-
?
additional information
?
-
-
substrate specificity of EII and mutant Hyb24, no activity with D-Ala-D-Ala
-
-
?
additional information
?
-
enzyme 6-aminohexanoate-dimer hydrolase catalyzes amide synthesis. The yield of this reverse reaction in 90% t-butyl alcohol varies drastically when enzyme mutants with substitutions of several amino acids located at the entrance of the catalytic cleft are used. The shift of the internal equilibrium between the enzyme-substrate complex and enzyme-product complex by the water-excluding effect alters the rate of the forward and reverse reactions. The local hydrophobic environment potentially provides a reaction center suitable for efficient amide synthesis
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?
