Literature summary for 3.5.1.46 extracted from

Ohki, T.; Mizuno, N.; Shibata, N.; Takeo, M.; Negoro, S.; Higuchi, Y.
Crystallization and X-ray diffraction analysis of 6-aminohexanoate-dimer hydrolase from Arthrobacter sp. KI72 (2005), Acta Crystallogr. Sect. F, 61, 928-930.

Cloned(Commentary)

Cloned (Comment)	Organism
nylB24 gene, expression of the His-tagged EII-EII'-hybrid Hyb24 in Escherichia coli strain KP3998	Arthrobacter sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant Hyb24, sitting drop vapor diffusion, 38 mg/ml protein in 20 mM phosphate, pH 7.3, and 10% glycerol, 0.02 ml of protein solution is mixed with the same volume of reservoir solution and equilibrated against 0.8 ml of reservoir solution, 10°C, 48 h, preparation of methylmercuric chloride heavy atom derivatives, X-ray diffraction structure determination and analysis at 1.75-1.8 A resolution	Arthrobacter sp.

Crystallization (Comment)

Organism

purified recombinant Hyb24, sitting drop vapor diffusion, 38 mg/ml protein in 20 mM phosphate, pH 7.3, and 10% glycerol, 0.02 ml of protein solution is mixed with the same volume of reservoir solution and equilibrated against 0.8 ml of reservoir solution, 10°C, 48 h, preparation of methylmercuric chloride heavy atom derivatives, X-ray diffraction structure determination and analysis at 1.75-1.8 A resolution

Arthrobacter sp.

Protein Variants

Protein Variants	Comment	Organism
T3A/P4R/T5S/S8Q/D15G	construction of a hybrid of isozymes EII and EII', termed Hyb24, by five amino acid replacement in EII', the mutant shows the same activity as EII'	Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-(6-aminohexanoyl)-6-aminohexanoate + H2O	Arthrobacter sp.	the enzyme is responsible for the degradation of nylon-6 industrial production by-products	6-aminohexanoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter sp.	-	formerly Flavobacterium sp., strain K172, enzyme form EII and cryptic enzyme form EII'	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged EII-EII'-hybrid Hyb24 from Escherichia coli strain KP3998 by anion exchange chromatography, gel filtration, and again anion exchange chromatography, to homogeneity	Arthrobacter sp.

Reaction

Reaction	Comment	Organism	Reaction ID
N-(6-aminohexanoyl)-6-aminohexanoate + H2O = 2 6-aminohexanoate	structure-function relationship	Arthrobacter sp.	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-(6-aminohexanoyl)-6-aminohexanoate + H2O	the enzyme is responsible for the degradation of nylon-6 industrial production by-products	Arthrobacter sp.	6-aminohexanoate	-	?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O	the enzyme hydrolyzes the linear dimer substrate	Arthrobacter sp.	6-aminohexanoate	-	?