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Literature summary for 3.5.1.46 extracted from
- Characterization of the 6-aminohexanoate-dimer hydrolase from Pseudomonas sp. NK87 (1993), J. Gen. Microbiol., 139, 787-795.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas sp. |
| expression in Escherichia coli | Flavobacterium sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl fluorophosphate | - |
Flavobacterium sp. |  |
| diisopropyl fluorophosphate | 80% inhibition at 1 mM | Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
Pseudomonas sp. | |
| 15 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
Flavobacterium sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Pseudomonas sp. | 5622 | - |
| intracellular | - |
Flavobacterium sp. | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE | Pseudomonas sp. |
| 42000 | - |
2 * 42000, SDS-PAGE | Flavobacterium sp. |
| 76000 | - |
gel filtration | Pseudomonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium sp. | - |
- |
- |
| Flavobacterium sp. KI72 | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
| Pseudomonas sp. NK87 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques, recombinant enzyme | Pseudomonas sp. |
| to homogeneity, chromatography techniques, recombinant enzyme | Flavobacterium sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Pseudomonas sp. | - |
| cell culture | - |
Flavobacterium sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.7 | - |
purified from parental strain | Pseudomonas sp. |
| 4 | - |
purified from cloned Escherichia coli strain | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-(6-aminohexanoyl)-6-aminohexanoate + H2O | - |
Pseudomonas sp. | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
| N-(6-aminohexanoyl)-6-aminohexanoate + H2O | - |
Flavobacterium sp. | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
| N-(6-aminohexanoyl)-6-aminohexanoate + H2O | - |
Flavobacterium sp. KI72 | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
| N-(6-aminohexanoyl)-6-aminohexanoate + H2O | - |
Pseudomonas sp. NK87 | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
| N-6-aminohexanoate pentamer + H2O | - |
Pseudomonas sp. | 6-aminohexanoate + ? | - |
? | |
| N-6-aminohexanoate pentamer + H2O | - |
Flavobacterium sp. | 6-aminohexanoate + ? | - |
? | |
| N-6-aminohexanoate pentamer + H2O | - |
Flavobacterium sp. KI72 | 6-aminohexanoate + ? | - |
? | |
| N-6-aminohexanoate pentamer + H2O | - |
Pseudomonas sp. NK87 | 6-aminohexanoate + ? | - |
? | |
| N-6-aminohexanoate tetramer + H2O | - |
Flavobacterium sp. | 6-aminohexanoate + N-6-aminohexanoate trimer | - |
? | |
| N-6-aminohexanoate tetramer + H2O | highest relative activity | Pseudomonas sp. | 6-aminohexanoate + N-6-aminohexanoate trimer | major products, the trimer is subsequently subjected to hydrolysis, digests exogenously linear oligomers from the N-terminus as monomeric units | ? | |
| N-6-aminohexanoate tetramer + H2O | - |
Flavobacterium sp. KI72 | 6-aminohexanoate + N-6-aminohexanoate trimer | - |
? | |
| N-6-aminohexanoate tetramer + H2O | highest relative activity | Pseudomonas sp. NK87 | 6-aminohexanoate + N-6-aminohexanoate trimer | major products, the trimer is subsequently subjected to hydrolysis, digests exogenously linear oligomers from the N-terminus as monomeric units | ? | |
| N-6-aminohexanoate trimer + H2O | - |
Pseudomonas sp. | 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
| N-6-aminohexanoate trimer + H2O | - |
Flavobacterium sp. | 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
| N-6-aminohexanoate trimer + H2O | - |
Flavobacterium sp. KI72 | 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
| N-6-aminohexanoate trimer + H2O | - |
Pseudomonas sp. NK87 | 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, SDS-PAGE | Pseudomonas sp. |
| dimer | 2 * 42000, SDS-PAGE | Flavobacterium sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EII | - |
Pseudomonas sp. |
| EII | - |
Flavobacterium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Flavobacterium sp. |
| 48 | - |
- |
Pseudomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable up to | Pseudomonas sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.2 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
Pseudomonas sp. | |
| 19 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
Flavobacterium sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pseudomonas sp. |
| 9 | - |
- |
Flavobacterium sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.5 | stable within | Pseudomonas sp. |
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