3.4.24.67: choriolysin H
This is an abbreviated version!
For detailed information about choriolysin H, go to the full flat file.
Word Map on EC 3.4.24.67
Reaction
Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-/-7-(4-methyl)coumarylamide =
Synonyms
chorionase, embryo-specific hatching enzyme, FHCE1, FHCE2, hatching enzyme, HCE, HCE-1, HCE1, HCE2, High choriolytic enzyme, medaka hatching enzyme, MHCE, More, PHE, Teleost hatching enzyme (component), ZHE1
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Substrates Products on EC 3.4.24.67 - choriolysin H
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Benzoyl-Arg 4-methylcoumarin 7-amide + H2O
?
-
hydrolysis at about 3% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin 7-amide + H2O
?
-
about 8.5% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
benzyloxycarbonyl-Phe + Arg-4-methylcoumarin 7-amide
-
-
-
-
?
Benzyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O
?
-
hydrolysis at about 2% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
benzyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O
?
-
preferred by HCE1
-
-
?
GSKTPQDPTQQ + H2O
GSKTPQ + DPTQQ
-
location: Q39/D40 in ChgL (ZI-3). Specific activity: 28.5 nmol/30min/microgram enzyme
-
-
?
KLMLKAPEPF + H2O
KLMLK + APEPF
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 7.6% of the rate with TVQQSDYLIK
-
-
?
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
hydrolysis at about 12% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
PGKNPNTPPIG + H2O
PGKNPN + TPPIG
-
location: N220/T221 in ChgH (ZI-1,2). Specific activity: 11.3 nmol/30min/microgram enzyme
-
-
?
PLPVRVEEVV + H2O
PLPVR + VEEVV
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 38% of the rate with TVQQSDYLIK
-
-
?
SAPVVRTSQAV + H2O
NPQVPQ + YPSKPQ
-
synthetic substrate generated from the 25 kDa product in hatching liquid with an inefficient cleavage site
-
-
?
Succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide + H2O
?
-
hydrolysis at about 13% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
Succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide + H2O
?
-
hydrolysis at about 45% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
succinyl-Ala-Pro-Ala-4-methylcoumaryl-7-amide + H2O
?
-
best substrate for both isoforms of HCE
-
-
?
succinyl-Ile-Ile-Trp-4-methylcoumarin 7-amide + H2O
?
-
about 5.7% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
Succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
best substrate of peptidyl 4-methylcoumarin 7-amides
-
-
?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide + H2O
?
-
about 40% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide + H2O
?
-
preferred by HCE2
-
-
?
t-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumarin 7-amide + H2O
?
-
about 9% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
TVQQSDYLIK + H2O
TVQQS + DYLIK
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein
-
-
?
VPFELRYPVPA + H2O
VPFELR + YPVPA
-
specific activity: 0.05 nmol/min/microgram protein
-
-
?
ZP2 + H2O
?
-
zebrafish egg envelope glycoprotein, cleavage at Ser47/Asp48 and Arg66/Val67 in the unfertilized egg, at Gln79/Ala80 in the fertilized egg
-
-
?
ZP3 + H2O
?
-
zebrafish egg envelope glycoprotein, cleavage at Gln79/Ala80 in the unfertilized egg, at Gln67/Ala68 in the fertilized egg
-
-
?
chorion + H2O
?
-
concerted action with other component of hatching enzyme, i.e. choriolysin L, EC 3.4.24.66
-
-
?
chorion + H2O
?
-
specific substrate, the hatching enzyme is secreted from embryos for digesting their protective extracellular coats
-
-
?
Chorion + H2O
Swollen chorion + ?
-
enzyme binds stoichiometrically to chorion (0.012 mg HCE/20 mg chorion) and remains bound to swollen chorion at pH 7.5 and is released at pH 10.2
-
?
Chorion + H2O
Swollen chorion + ?
-
i.e. fish egg envelope, hardened chorion of fertilized eggs, isolated from blastulae
swollen chorion + unique proline-rich polypeptides
?
EVLPLDNPPPA + H2O
EVLPLD + NPPPA
-
specific activity: 0.98 nmol/min/microgram protein
-
-
?
EVLPLDNPPPA + H2O
EVLPLD + NPPPA
-
specific activity: 1.07 nmol/min/microgram protein
-
-
?
NPQVPQYPSKPQ + H2O
NPQVPQ + YPSKPQ
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 17% of the rate with TVQQSDYLIK
-
-
?
NPQVPQYPSKPQ + H2O
NPQVPQ + YPSKPQ
-
Pro-Xaa-Yaa repeat sequence ZI-1,2. Specific activity: 43.7 nmol/30min/microgram enzyme
-
-
?
NPSYPQNPSYPQ + H2O
NPSYPQ + NPSYPQ
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 32% of the rate with TVQQSDYLIK
-
-
?
NPSYPQNPSYPQ + H2O
NPSYPQ + NPSYPQ
-
Pro-Xaa-Yaa repeat sequence ZI-1,2. Specific activity: 81.7 nmol/30min/microgram enzyme
-
-
?
PSKRPEAPGVP + H2O
PSKRPE + APGVP
-
specific activity: 0.95 nmol/min/microgram protein
-
-
?
PSKRPEAPGVP + H2O
PSKRPE + APGVP
-
specific activity: 1.4 nmol/min/microgram protein
-
-
?
QTPSYPQQ + H2O
QTPS + YPQQ
-
specific activity: 11.6 nmol/min/microgram protein
-
-
?
QTPSYPQQ + H2O
QTPS + YPQQ
-
specific activity: 13.7 nmol/min/microgram protein
-
-
?
SKPQYPNP + H2O
SKPQ + YPNP
-
specific activity: 16.0 nmol/min/microgram protein
-
-
?
SKPQYPNP + H2O
SKPQ + YPNP
-
specific activity: 23.2 nmol/min/microgram protein
-
-
?
YPSKPQTPTET + H2O
YPSKPQ + TPTET
-
specific activity: 0.35 nmol/min/microgram protein
-
-
?
YPSKPQTPTET + H2O
YPSKPQ + TPTET
-
specific activity: 0.66 nmol/min/microgram protein
-
-
?
additional information
?
-
-
isoform ZHE1 cleaves the peptide bonds at the C-terminal side of Arg, Tyr, Asn, Trp, Ala, Asp, Phe and Gly
-
-
?
additional information
?
-
-
cleavage sites of FHCE and FLCE on egg envelope subunit proteins are determined by comparing the N-terminal amino acid sequences of digests with the sequences deduced from the cDNAs for egg envelope subunit proteins. FHCE and FLCE cleave different sites of the subunit proteins. FHCE efficiently cleaves the Pro-X-Y repeat regions into tripeptides to dodecapeptides to swell the envelope
-
-
?
additional information
?
-
-
unfertilized egg envelope (UFE) is digested by one of the enzymes FHCE1/2 or FLCE
-
-
?
additional information
?
-
-
no substrates are chorion of unfertilized eggs
-
-
?
additional information
?
-
-
exhibits choriolytic and proteolytic activity
-
-
?
additional information
?
-
-
N-tert-butoxycarbonyl-Ile-Glu-Gly-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Ser-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Glu-Lys-Lys 4-methylcoumarin 7-amide, succinyl-Gly-Pro 4-methylcoumarin 7-amide or Leu 4-methylcoumarin 7-amide
-
-
?
additional information
?
-
-
cleavage sites of HCE and LCE on the egg envelope that are primarily constructed of two groups of subunit proteins, ZI-1,2 and ZI-3, are determined. HCE cleaves the N-terminal region of ZI-1,2 and ZI-3, mainly the Pro-Xaa-Yaa repeat sequence of ZI-1,2 into hexapeptides, but not the site within a zona pellucida (ZP) domain that is considered to be the core structure of the egg envelope. The cleavage of the N-terminal regions results in swelling and softening of the envelope
-
-
?
additional information
?
-
-
the enzyme is a trypsin-type serine protease
-
-
?
