3.4.24.67: choriolysin H

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For detailed information about choriolysin H, go to the full flat file.

Word Map on EC 3.4.24.67

3.4.24.67

urchin

oryzias

latipes

astacin

blastula

teleostean

animal-vegetal

euteleosts

pre-hatching

trypsin-type

analysis

degradation

Reaction

Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-/-7-(4-methyl)coumarylamide =

Synonyms

chorionase, embryo-specific hatching enzyme, FHCE1, FHCE2, hatching enzyme, HCE, HCE-1, HCE1, HCE2, High choriolytic enzyme, medaka hatching enzyme, MHCE, More, PHE, Teleost hatching enzyme (component), ZHE1

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.24 Metalloendopeptidases

3.4.24.67 choriolysin H

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Results	in table
8	AA Sequence
3	Application
1	CAS Registry Number
9	Cloned(Commentary)
1	Crystallization (Commentary)
1	Expression
3	General Information
24	Inhibitors
2	KM Value [mM]
4	Localization
12	Metals/Ions
16	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
20	Organism
1	PDB ID
8	pH Optimum
3	pH Range
10	Purification (Commentary)
1	Reaction
1	Reaction Type
19	Reference
31	Source Tissue
3	Specific Activity [micromol/min/mg]
1	Storage Stability
67	Substrates and Products (Substrate)
5	Subunits
34	Synonyms
6	Temperature Optimum [°C]
1	Temperature Range [°C]
5	Temperature Stability [°C]

Purification

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Purification on EC 3.4.24.67 - choriolysin H

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2 entries

2 isomers: HCE-1 and HCE-2, separable by HPLC ion exchange chromatography

Oryzias latipes

31416

cationic column chromatography

from hatching liquid

gel filtration, cation exchange column chromatography

Fundulus heteroclitus

668554

native enzyme 40fold from embryos by gel filtration and anion exchange chromatography

Paralichthys olivaceus

partial

partly purified by cation-exchange column chromatography and gel filtration, two isoforms

Oryzias latipes

668840