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Literature summary for 3.4.24.67 extracted from

  • Yasumasu, S.; Kawaguchi, M.; Ouchi, S.; Sano, K.; Murata, K.; Sugiyama, H.; Akema, T.; Iuchi, I.
    Mechanism of egg envelope digestion by hatching enzymes, HCE and LCE in medaka, Oryzias latipes (2010), J. Biochem., 148, 439-448.
    View publication on PubMed
Search for more literature for 3.4.24.67

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
480
-
 NPQVPQYPSKPQ pH 8.0, 30°C Oryzias latipes

Organism

Organism UniProt Comment Textmining
Oryzias latipes
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Oryzias latipes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSKTPQDPTQQ + H2O location: Q39/D40 in ChgL (ZI-3). Specific activity: 28.5 nmol/30min/microgram enzyme Oryzias latipes GSKTPQ + DPTQQ
-
 ?
additional information cleavage sites of HCE and LCE on the egg envelope that are primarily constructed of two groups of subunit proteins, ZI-1,2 and ZI-3, are determined. HCE cleaves the N-terminal region of ZI-1,2 and ZI-3, mainly the Pro-Xaa-Yaa repeat sequence of ZI-1,2 into hexapeptides, but not the site within a zona pellucida (ZP) domain that is considered to be the core structure of the egg envelope. The cleavage of the N-terminal regions results in swelling and softening of the envelope Oryzias latipes ?
-
 ?
NPQVPQYPSKPQ + H2O Pro-Xaa-Yaa repeat sequence ZI-1,2. Specific activity: 43.7 nmol/30min/microgram enzyme Oryzias latipes NPQVPQ + YPSKPQ
-
 ?
NPSYPQNPSYPQ + H2O Pro-Xaa-Yaa repeat sequence ZI-1,2. Specific activity: 81.7 nmol/30min/microgram enzyme Oryzias latipes NPSYPQ + NPSYPQ
-
 ?
PGKNPNTPPIG + H2O location: N220/T221 in ChgH (ZI-1,2). Specific activity: 11.3 nmol/30min/microgram enzyme Oryzias latipes PGKNPN + TPPIG
-
 ?
SAPVVRTSQAV + H2O synthetic substrate generated from the 25 kDa product in hatching liquid with an inefficient cleavage site Oryzias latipes NPQVPQ + YPSKPQ
-
 ?

Synonyms

Synonyms Comment Organism
HCE
-
 Oryzias latipes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryzias latipes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Oryzias latipes