3.4.24.27: thermolysin
This is an abbreviated version!
For detailed information about thermolysin, go to the full flat file.
Word Map on EC 3.4.24.27
-
3.4.24.27
-
chymotrypsin
-
elastase
-
metalloprotease
-
subtilisin
-
staphylococcus
-
edman
-
pepsin
-
aureus
-
carboxypeptidase
-
endopeptidase
-
bromide
-
cyanogen
-
collagenase
-
proteinases
-
dipeptide
-
angiotensin
-
pronase
-
metalloproteinases
-
alpha-chymotrypsin
-
thermolytic
-
hydrolysates
-
metalloendopeptidase
-
stearothermophilus
-
endoproteinase
-
phosphoramidon
-
i-converting
-
enkephalinase
-
rhodopsin
-
2-macroglobulin
-
3.4.24.11
-
cell-binding
-
alcalase
-
ace-inhibitory
-
hexxh
-
dispase
-
aspartame
-
thiorphan
-
neprilysin
-
s-carboxymethylated
-
half-cystine
-
astacin
-
synthesis
-
industry
-
nutrition
-
food industry
-
diagnostics
-
medicine
-
analysis
- 3.4.24.27
- chymotrypsin
- elastase
- metalloprotease
- subtilisin
- staphylococcus
-
edman
- pepsin
- aureus
- carboxypeptidase
- endopeptidase
- bromide
-
cyanogen
- collagenase
- proteinases
- dipeptide
- angiotensin
- pronase
- metalloproteinases
- alpha-chymotrypsin
-
thermolytic
- hydrolysates
- metalloendopeptidase
- stearothermophilus
-
endoproteinase
- phosphoramidon
-
i-converting
- enkephalinase
- rhodopsin
-
2-macroglobulin
-
3.4.24.11
-
cell-binding
- alcalase
-
ace-inhibitory
-
hexxh
-
dispase
- aspartame
- thiorphan
- neprilysin
-
s-carboxymethylated
-
half-cystine
- astacin
- synthesis
- industry
- nutrition
- food industry
- diagnostics
- medicine
- analysis
Reaction
preferential cleavage: -/-Leu > -/-Phe =
Synonyms
Bacillus thermoproteolyticus neutral proteinase, EC 3.4.24.4, hspA, LIC13322, Neutral metalloproteinase, NprM, protease type X, proteinase type X, Proteinase, Bacillus thermoproteolyticus neutral, protex 14L, Thermoase, thermoase PC10F, Thermoase Y10, thermolysin, thermolysin-like protease, Thermostable neutral proteinase, TL, TLN, TLP, TLP-ste
ECTree
3.4.24.27 thermolysinAdvanced search results
results (
results (Turnover Number
Turnover Number on EC 3.4.24.27 - thermolysin
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
2.36
(7-methoxycoumarin-4yl) acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diamino-propionyl]-L-Ala-L-Arg-NH2
-
pH and temperature not specified in the publication
2.6
(europium(III) complex of a modified terpyridine)-K1K2K2GFSAK2K-black hole quencher 2
-
pH and temperature not specified in the publication
-
0.7
5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2
-
pH 7.4, 30°C
-
5.7
5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2
-
pH 7.4, 30°C
-
7.82
5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2
-
pH 7.4, 30°C
861 - 951
Benzyloxycarbonyl-(4-nitro)Phe-Leu-Ala
-
depending on mode of preparation of the form of enzyme
407 - 968
Benzyloxycarbonyl-Gly-Phe-Leu-Ala
-
depending on mode of preparation of the form of enzyme
362 - 605
Benzyloxycarbonyl-Phe-Leu-Ala
-
depending on mode of preparation of the form of enzyme
6.9
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.3
L-phenylalanine methyl ester
-
mutant enzyme F114A, at pH 7.5, at 25°C
1.3
L-phenylalanine methyl ester
-
wild type enzyme, at pH 7.5, at 25°C
2.1
L-phenylalanine methyl ester
-
mutant enzyme D150A, at pH 7.5, at 25°C
2.3
L-phenylalanine methyl ester
-
mutant enzyme N227H, at pH 7.5, at 25°C
2.5
L-phenylalanine methyl ester
-
mutant enzyme D150E, at pH 7.5, at 25°C
3.2
L-phenylalanine methyl ester
-
mutant enzyme D150W, at pH 7.5, at 25°C
4.9
L-phenylalanine methyl ester
-
mutant enzyme I168A, at pH 7.5, at 25°C
13.58
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155A/I156V, pH 7.0, 37°C
26.67
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155A/I156N, pH 7.0, 37°C
48.33
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155S/I156N, pH 7.0, 37°C
51.67
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant wild-type enzyme, pH 7.0, 37°C
80
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155A, pH 7.0, 37°C
105
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155S/I156V, pH 7.0, 37°C
165
N-benzyloxycarbonyl-Gly-L-Leu amide
-
recombinant mutant L155S, pH 7.0, 37°C
4.8
N-benzyloxycarbonyl-L-Asp-L-Phe-methyl ester
-
recombinant mutant G117R, pH 7.5, 25°C
7.9
N-benzyloxycarbonyl-L-Asp-L-Phe-methyl ester
-
recombinant wild-type enzyme, pH 7.5, 25°C
8.4
N-benzyloxycarbonyl-L-Asp-L-Phe-methyl ester
-
recombinant mutant G117K, pH 7.5, 25°C
10.5
N-benzyloxycarbonyl-L-Asp-L-Phe-methyl ester
-
recombinant mutant G117E, pH 7.5, 25°C
1.3
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, recombinant mutant Q225A
1.6
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, recombinant mutant Q225V
3.3
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.0, 25°C, recombinant mutant G8C/N60C/S65P
3.3
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, recombinant mutant Q225D
3.3
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme S53D/L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
3.4
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.0, 25°C, recombinant wild-type enzyme
3.6
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, recombinant mutant Q225R
3.7
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, recombinant mutant Q128K
3.8
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, recombinant enzyme
3.8
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, recombinant wild-type enzyme
3.8
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, recombinant mutant Q128A
4.3
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, native enzyme
4.3
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, native wild-type enzyme
4.3
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, recombinant mutants Q128E and Q225E
4.7
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.5, 25°C, recombinant wild-type enzyme and mutant Q225K
5.4
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
5.9
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
wild type enzyme, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
6.3
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme L155A/G8C/N60C/S65P, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
6.4
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme S53D/L155A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
6.7
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.0, 25°C, recombinant mutant L144S
7
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
pH 7.0, 25°C, recombinant mutant G8C/N60C/S65P/L144S
7.4
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme S53D/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
7.7
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
8.7
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
9.4
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme L144S, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
15.5
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme D150E/I168A, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
15.6
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme L144S/D150E/S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
16.5
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
17
N-carbobenzoxy-L-Asp-L-Phe methyl ester
-
mutant enzyme L144S/D150E, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
1.5
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme F114A, at pH 7.5, at 25°C
9.5
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
wild type enzyme, at pH 7.5, at 25°C
12.3
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme I168A, at pH 7.5, at 25°C
17.1
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme N227H, at pH 7.5, at 25°C
20.5
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme D150E, at pH 7.5, at 25°C
24.4
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme D150A, at pH 7.5, at 25°C
49.4
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
-
mutant enzyme D150W, at pH 7.5, at 25°C
0.3
N-carbobenzoxy-L-aspartic acid
-
mutant enzyme F114A, at pH 7.5, at 25°C
1.3
N-carbobenzoxy-L-aspartic acid
-
wild type enzyme, at pH 7.5, at 25°C
2.1
N-carbobenzoxy-L-aspartic acid
-
mutant enzyme D150A, at pH 7.5, at 25°C
2.3
N-carbobenzoxy-L-aspartic acid
-
mutant enzyme N227H, at pH 7.5, at 25°C
2.5
N-carbobenzoxy-L-aspartic acid
-
mutant enzyme D150E, at pH 7.5, at 25°C
3.2
N-carbobenzoxy-L-aspartic acid
-
mutant enzyme D150W, at pH 7.5, at 25°C
4.9
N-carbobenzoxy-L-aspartic acid
-
mutant enzyme I168A, at pH 7.5, at 25°C
5.4
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
purified recombinant mutant N116T, pH 7.5, 25°C
6.7
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
purified recombinant mutant N116A, pH 7.5, 25°C
7.1
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
purified recombinant mutant N116Q, pH 7.5, 25°C
7.7
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
purified recombinant wild-type enzyme, pH 7.5, 25°C
12
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
purified recombinant mutant N116D, pH 7.5, 25°C
additional information
additional information
-
effect of various n-pentanal concentrations of turnover number
-
additional information
additional information
-
overview: turnover number of synthetic oligopeptides
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