KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
5,6-carboxyfluorescein-PAGLAC(Cy5)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
0.0116 | - |
5,6-carboxyfluorescein-PVAGLAGC(Cy5)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
0.0176 | - |
5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
0.0533 | - |
5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
0.0776 | - |
5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2 + H2O | - |
Geobacillus stearothermophilus | 5,6-carboxyfluorescein-PAG + LAC(Alexa Fluor 647)-NH2 | - |
? | |
5,6-carboxyfluorescein-PAGLAC(Cy5)-NH2 + H2O | - |
Geobacillus stearothermophilus | 5,6-carboxyfluorescein-PAG + LAC(Cy5)-NH2 | - |
? | |
5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2 + H2O | - |
Geobacillus stearothermophilus | 5,6-carboxyfluorescein-PSVAG + LAGGC(Alexa Fluor 647)-NH2 | - |
? | |
5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2 + H2O | - |
Geobacillus stearothermophilus | 5,6-carboxyfluorescein-PSVAG + LAGGC(Cy5)-NH2 | - |
? | |
5,6-carboxyfluorescein-PVAGLAGC(Cy5)-NH2 + H2O | - |
Geobacillus stearothermophilus | 5,6-carboxyfluorescein-PVAG + LAGC(Cy5)-NH2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
5,6-carboxyfluorescein-PAGLAC(Cy5)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
0.7 | - |
5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
2.96 | - |
5,6-carboxyfluorescein-PVAGLAGC(Cy5)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
5.7 | - |
5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
7.82 | - |
5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.1 | - |
5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
73.5 | - |
5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
154.2 | - |
5,6-carboxyfluorescein-PAGLAC(Cy5)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
255.2 | - |
5,6-carboxyfluorescein-PVAGLAGC(Cy5)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus | |
444.3 | - |
5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2 | pH 7.4, 30°C | Geobacillus stearothermophilus |