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3.4.21.70: Pancreatic endopeptidase E

This is an abbreviated version!
For detailed information about Pancreatic endopeptidase E, go to the full flat file.

Word Map on EC 3.4.21.70

Reaction

Preferential cleavage: Ala-/-. Does not hydrolyse elastin =

Synonyms

CBPP, Cholesterol-binding pancreatic proteinase, Cholesterol-binding proteinase, Cholesterol-binding serine proteinase, pancreatic endopeptidase E, Pancreatic protease E, Pancreatic proteinase E, PEE, Proteinase, cholesterol-binding serine, Proteinase, E, Proteinase, pancreas E

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.70 Pancreatic endopeptidase E

Storage Stability

Storage Stability on EC 3.4.21.70 - Pancreatic endopeptidase E

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, slow inactivation over a period of a month to a limiting value of around 75% of maximal activity, this decrease is most rapid at pH 3 and slowest at pH 9
-
22°C, 50 mM Tris/100 mM NaCl, pH 7.9, 15 mM NaN3, with or without 10 mM CaCl2, loss of approximately 8% of its esterolytic activity per week
-
24°C, dilute solution of protein concentration of less than 0.4 mg/ml, pH 4.0-8.0, stable for 3 months
-
4°C, stable over months without loss of activity
-