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Literature summary for 3.4.21.70 extracted from

  • Kobayashi, R.; Kobayashi, Y.; Hirs, C.H.W.
    The specificity of porcine pancreatic protease E (1981), J. Biol. Chem., 256, 2460-2465.
    View publication on PubMed
Search for more literature for 3.4.21.70

General Stability

General Stability Organism
Ca2+ at concentrations up to 0.4 M has no effect on stability Sus scrofa
Loses up to 20% of activity when subjected at 0.2 mg protein/ml to lyophilization or freezing and thawing Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.86
-
 Acetyl-tri-L-alanine methyl ester
-
 Sus scrofa
0.95
-
 Succinyl-tri-L-alanine 4-nitroanilide
-
 Sus scrofa
24
-
 Benzoyl-L-alanine methyl ester
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sus scrofa the specificity is complementary to that of the chymotrypsins, this property may be physiologically significant ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Sus scrofa
-

Storage Stability

Storage Stability Organism
0°C, slow inactivation over a period of a month to a limiting value of around 75% of maximal activity, this decrease is most rapid at pH 3 and slowest at pH 9 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acetyl-tri-L-alanine methyl ester + H2O
-
 Sus scrofa Acetyl-tri-L-alanine + methanol
-
 ?
Benzoyl-L-alanine methyl ester + H2O
-
 Sus scrofa Benzoyl-L-alanine + methanol
-
 ?
Insulin A-chain + H2O enzyme acts on the carbonyl bonds of Ala8, Val10 and Ser12, with less extensive action at Ile2 or Val3, Ser9, Leu13, Tyr14, Glu15, and Tyr19 Sus scrofa Hydrolyzed insulin A-chain
-
 ?
Insulin B-chain + H2O enzyme acts on the carbonyl bonds of Ser9, Val12, Ala14 and Val18 with less extensive action at Val2, Leu15, and Gly23, and minor cleavages at Leu6, Leu11, and Tyr16 Sus scrofa Hydrolyzed insulin B-chain
-
 ?
additional information pronounced selectivity for the carbonyl bonds of serine, threonine, alanine, and valine residues, with the latter most favoured Sus scrofa ?
-
 ?
additional information the specificity is complementary to that of the chymotrypsins, this property may be physiologically significant Sus scrofa ?
-
 ?
Succinyl-tri-L-alanine 4-nitroanilide + H2O
-
 Sus scrofa Succinyl-tri-L-alanine + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3
-
 Benzoyl-L-alanine methyl ester
-
 Sus scrofa
7.5
-
 Succinyl-tri-L-alanine 4-nitroanilide
-
 Sus scrofa
59
-
 Acetyl-tri-L-alanine methyl ester
-
 Sus scrofa