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Literature summary for 3.4.21.70 extracted from
- A novel proteinase from human pancreas (1984), Biochem. J., 219, 735-742.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl phosphofluoridate | - |
Homo sapiens |  |
| phenylmethanesulfonyl fluoride | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04706 | - |
Benzyloxycarbonylalanine 4-nitrophenyl ester | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
1 * 28000, human, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 22°C, 50 mM Tris/100 mM NaCl, pH 7.9, 15 mM NaN3, with or without 10 mM CaCl2, loss of approximately 8% of its esterolytic activity per week | Homo sapiens |
| 4°C, stable over months without loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzyloxycarbonylalanine 4-nitrophenyl ester + H2O | - |
Homo sapiens | Benzyloxycarbonylalanine + 4-nitrophenol | - |
? | |
| Chymotrypsin + H2O | - |
Homo sapiens | Hydrolyzed chymotrypsin | - |
? | |
| Elastase + H2O | - |
Homo sapiens | Hydrolyzd elastase | - |
? | |
| Glucagon + H2O | carbonyl bond of Ala is the favoured cleavage site | Homo sapiens | ? | - |
? | |
| additional information | chromogenic substrates | Homo sapiens | ? | - |
? | |
| Trypsin + H2O | - |
Homo sapiens | Hydrolyzed trypsin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, human, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 41 | - |
cleavage of benzyloxycarbonylalanine 4-nitrophenyl ester | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
loss of activity within 5 min | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
benzyloxycarbonylalanine 4-nitrophenyl ester | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
relatively resistant towards acid, the activity decreases by 20% by incubation in 50 mM acetic acid and by 60% in 50 mM HCl, complete inactivation by treatment with 50 mM NaOH | Homo sapiens |
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Release 2023.1 (January 2023)
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