3.2.2.2: inosine nucleosidase

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For detailed information about inosine nucleosidase, go to the full flat file.

Reaction

Synonyms

guanosine-inosine-preferring nucleoside N-ribohydrolase, inosinase, inosine hydrolase, inosine-adenosine-guanosine nucleoside hydrolase, inosine-guanosine nucleosidase, NSH2, nucleosidase, inosine, purine-specific nucleoside hydrolase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.2 Hydrolysing N-glycosyl compounds

                3.2.2.2 inosine nucleosidase

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Results	in table
16	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
1	General Information
1	General Stability
6	IC50 Value
24	Inhibitors
20	KM Value [mM]
3	Localization
2	Metals/Ions
5	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
57	Organism
5	Pathway
8	pH Optimum
3	pH Range
1	pI Value
2	Protein Variants
6	Purification (Commentary)
1	Reaction
1	Reaction Type
17	Reference
19	Source Tissue
7	Specific Activity [micromol/min/mg]
4	Storage Stability
51	Substrates and Products (Substrate)
3	Subunits
9	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Range [°C]
5	Temperature Stability [°C]
4	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 3.2.2.2 - inosine nucleosidase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

100

Saccharolobus solfataricus

-

half-live 45 min

695873

105

Saccharolobus solfataricus

-

half-live 30 min

695873

110

Saccharolobus solfataricus

-

half-live 15 min

695873

50 - 60

Penicillium viridicatum

-

the enzyme shows 68.18%, 45.45% and 28.78% residual activities for inosine hydrolysis after 4 min, 6 min, and 8 min of incubation, respectively, at 50°C. The enzyme is completely inactivated after 10 min at 50°C and 4 min at 60°C

713705

90

Saccharolobus solfataricus

-

100% activity after 1h, apparent melting temperature: 107°C

695873