3.2.1.193: ginsenosidase type I
This is an abbreviated version!
For detailed information about ginsenosidase type I, go to the full flat file.
Word Map on EC 3.2.1.193
-
3.2.1.193
-
ginseng
-
aspergillus
-
protopanaxadiol
-
paecilomyces
-
bioreactor
-
panax
-
glycoside
-
mucilaginosus
-
paenibacillus
-
niger
-
synthesis
- 3.2.1.193
- ginseng
- aspergillus
- protopanaxadiol
- paecilomyces
-
bioreactor
- panax
- glycoside
- mucilaginosus
-
paenibacillus
- niger
- synthesis
Reaction
Synonyms
beta-glucosidase, BG-1, Bgl, BglPm, bgy2, ginsenosidase type I, ginsenosidase type-I, ginsenoside Rc-hydrolyzing beta-glucosidase, ginsenoside-hydrolyzing beta-glucosidase, LVISKB_0241, More
ECTree
3.2.1.193 ginsenosidase type IAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.1.193 - ginsenosidase type I
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ginsenoside Rb1 + H2O
ginsenoside Rh2 + 3 D-glucose
-
via ginsenoside F2
-
-
?
ginsenoside Rb2 + H2O
ginsenoside Rh2 + 2 D-glucose + L-arabinopyranose
-
via ginsenoside F2
-
-
?
ginsenoside Rc + H2O
ginsenoside Rh2 + 2 D-glucose + L-arabinofuranose
-
via ginsenoside F2
-
-
?
ginsenoside Rd + H2O
ginsenoside + D-glucose
-
ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside
-
-
?
2-nitrophenyl-beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
93% activity compared to 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
2-nitrophenyl-beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
93% activity compared to 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
4-nitrophenyl-alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
30% activity compared to 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
4-nitrophenyl-alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
Armillaria mellea KACC 50013
-
30% activity compared to 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + L-arabinopyranose
-
10% activity compared to 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + L-arabinopyranose
Armillaria mellea KACC 50013
-
10% activity compared to 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
4-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
-
?
4-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
?
4-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenosides Rd and F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
Aspergillus niger g.848
-
via ginsenosides Rd and F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenoside F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenosides F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenosides F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenosides F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenosides F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenoside F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenoside F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenosides F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenosides F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenosides F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenoside F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenosides F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
via ginsenosides F2
-
-
?
ginsenoside Rb1 + H2O
ginsenoside F2 + 2 D-glucose
-
ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-glucoside
-
-
?
ginsenoside Rb1 + H2O
ginsenoside F2 + 2 D-glucose
-
ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-glucoside
-
-
?
ginsenoside Rb1 + H2O
ginsenoside F2 + 2 D-glucose
-
-
-
-
?
ginsenoside Rb1 + H2O
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
ginsenoside Rd + D-glucose
-
-
-
-
?
ginsenoside Rb2 + H2O
ginsenoside C-K + 2 D-glucose + L-arabinofuranose
-
-
-
-
?
ginsenoside Rb2 + H2O
ginsenoside C-K + 2 D-glucose + L-arabinofuranose
-
-
-
-
?
ginsenoside Rb2 + H2O
ginsenoside C-K + 2 D-glucose + L-arabinopyranose
-
via ginsenosides Rd and F2
-
-
?
ginsenoside Rb2 + H2O
ginsenoside C-K + 2 D-glucose + L-arabinopyranose
Aspergillus niger g.848
-
via ginsenosides Rd and F2
-
-
?
ginsenoside Rb2 + H2O
ginsenoside C-K + 2 D-glucose + L-arabinopyranose
-
via ginsenoside F2
-
-
?
ginsenoside Rb2 + H2O
ginsenoside C-K + 2 D-glucose + L-arabinopyranose
-
via ginsenoside F2
-
-
?
ginsenoside Rb2 + H2O
ginsenoside F2 + D-glucose + L-arabinopyranose
-
-
-
-
?
ginsenoside Rb2 + H2O
ginsenoside F2 + D-glucose + L-arabinopyranose
-
ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinopyranoside
-
-
?
ginsenoside Rb2 + H2O
ginsenoside F2 + D-glucose + L-arabinopyranose
-
ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinopyranoside
-
-
?
ginsenoside Rb2 + H2O
ginsenoside F2 + D-glucose + L-arabinopyranose
-
-
-
-
?
ginsenoside Rb2 + H2O
ginsenoside F2 + D-glucose + L-arabinopyranose
-
-
-
-
?
ginsenoside Rb3 + H2O
ginsenoside C-K + 2 D-glucose + xylose
-
via ginsenoside F2
-
-
?
ginsenoside Rb3 + H2O
ginsenoside C-K + 2 D-glucose + xylose
Aspergillus niger g.848
-
via ginsenoside F2
-
-
?
ginsenoside Rb3 + H2O
ginsenoside F2 + D-glucose + D-xylose
-
ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-xyloside
-
-
?
ginsenoside Rb3 + H2O
ginsenoside F2 + D-glucose + D-xylose
-
ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-xyloside
-
-
?
ginsenoside Rc + H2O
ginsenoside C-K + 2 D-glucose + L-arabinofuranose
-
via ginsenoside F2
-
-
?
ginsenoside Rc + H2O
ginsenoside C-K + 2 D-glucose + L-arabinofuranose
Aspergillus niger g.848
-
via ginsenoside F2
-
-
?
ginsenoside Rc + H2O
ginsenoside C-K + 2 D-glucose + L-arabinofuranose
-
via ginsenoside F2
-
-
?
ginsenoside Rc + H2O
ginsenoside C-K + 2 D-glucose + L-arabinofuranose
-
via ginsenoside F2
-
-
?
ginsenoside Rc + H2O
ginsenoside F2 + D-glucose + L-arabinofuranose
-
-
-
-
?
ginsenoside Rc + H2O
ginsenoside F2 + D-glucose + L-arabinofuranose
-
ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinofuranoside
-
-
?
ginsenoside Rc + H2O
ginsenoside F2 + D-glucose + L-arabinofuranose
-
ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinofuranoside
-
-
?
ginsenoside Rc + H2O
ginsenoside F2 + D-glucose + L-arabinofuranose
-
-
-
-
?
ginsenoside Rc + H2O
ginsenoside F2 + D-glucose + L-arabinofuranose
-
-
-
-
?
ginsenoside Rc + H2O
ginsenoside F2 + D-glucose + L-arabinofuranose
-
-
-
-
?
ginsenoside Rd + H2O
ginsenoside F2 + D-glucose
-
-
-
-
?
ginsenoside Rg3 + H2O
ginsenoside Rh2 + D-glucose
-
-
-
-
?
gypenoside XVII + H2O
F2 + D-glucose
-
GypXVI, enzyme mutants W512A and W512K hydrolyze the inner glucose linked to the C-3 position and the outer glucose linked to the C-20 position of GypXVII to produce GypLXXV (cf. EC 3.2.1.191) and F2. Mutant W512R hydrolyzes only the outer glucose linked to the C-20 position of GypXVII to produce F2. The wild-type enzyme hydrolyzes GypXVI only to gypenoside LXXV, GypLXXV
-
-
?
gypenoside XVII + H2O
F2 + D-glucose
-
GypXVI, enzyme mutants W512A and W512K hydrolyze the inner glucose linked to the C-3 position and the outer glucose linked to the C-20 position of GypXVII to produce GypLXXV (cf. EC 3.2.1.191) and F2. Mutant W512R hydrolyzes only the outer glucose linked to the C-20 position of GypXVII to produce F2. The wild-type enzyme hydrolyzes GypXVI only to gypenoside LXXV, GypLXXV
-
-
?
additional information
?
-
-
enzyme BG-1 hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site or outer beta-(1->2)-glucosidic linkage at the C-3 site of G-Rc to produce ginsenoside Rd and compound Mc1, respectively. The enzyme also hydrolyzes outer and inner glucosidic linkages at the C-3 site of ginsenoside Rd to produce compound K via ginsenoside F2, and inner glucosidic linkage at the C-3 site of compound Mc1 to produce ginsenoside compound Mc. Compound Mc is also slowly hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site to produce compound K with reaction time prolongation. Finally, the pathways for formation of C-Mc and C-K from G-Rc by enzyme BG-1 are G-Rc -> C-Mc1 -> C-Mc and G-Rc -> G-Rd -> G-F2 -> C-K, respectively. Compound identification by TLC, HPLC, NMR spectroscopic, and UPLC/Q-TOF-MS analysis, detailed overview. No activity with 4-nitrophenyl -alpha-D-glucopyranoside and 4-nitrophenyl-alpha- and -beta-galactopyranoside
-
-
?
additional information
?
-
Armillaria mellea KACC 50013
-
enzyme BG-1 hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site or outer beta-(1->2)-glucosidic linkage at the C-3 site of G-Rc to produce ginsenoside Rd and compound Mc1, respectively. The enzyme also hydrolyzes outer and inner glucosidic linkages at the C-3 site of ginsenoside Rd to produce compound K via ginsenoside F2, and inner glucosidic linkage at the C-3 site of compound Mc1 to produce ginsenoside compound Mc. Compound Mc is also slowly hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site to produce compound K with reaction time prolongation. Finally, the pathways for formation of C-Mc and C-K from G-Rc by enzyme BG-1 are G-Rc -> C-Mc1 -> C-Mc and G-Rc -> G-Rd -> G-F2 -> C-K, respectively. Compound identification by TLC, HPLC, NMR spectroscopic, and UPLC/Q-TOF-MS analysis, detailed overview. No activity with 4-nitrophenyl -alpha-D-glucopyranoside and 4-nitrophenyl-alpha- and -beta-galactopyranoside
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
substrates are notoginseng leaf ginsenosides
-
-
?
additional information
?
-
-
the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I
-
-
?
additional information
?
-
Aspergillus niger g.848
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
Aspergillus niger g.848
-
substrates are notoginseng leaf ginsenosides
-
-
?
additional information
?
-
Aspergillus niger g.848
-
the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
the enzyme can hydrolyze different glycoside of protopanaxadiol type ginsenosides, can hydrolyze the 3(carbon)-O-beta-glucoside of Rb1, Rb2, Rb3, Rc, Rd, can hydrolyze 20(carbon)-O-beta-glucoside of Rb1, 20-O-beta-xyloside of Rb3, 20-O-alpha-arabinoside(p) of Rb2 and 20-O-alpha-arabinoside(f) of Rc to produce mainly F2, compound-K (C-K) and small Rh2, but can not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
the enzyme can hydrolyze different glycoside of protopanaxadiol type ginsenosides, can hydrolyze the 3(carbon)-O-beta-glucoside of Rb1, Rb2, Rb3, Rc, Rd, can hydrolyze 20(carbon)-O-beta-glucoside of Rb1, 20-O-beta-xyloside of Rb3, 20-O-alpha-arabinoside(p) of Rb2 and 20-O-alpha-arabinoside(f) of Rc to produce mainly F2, compound-K (C-K) and small Rh2, but can not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
recombinant wild-type beta-glucosidase from Clavibacter michiganensis specifically hydrolyzes the outer and inner glucose linked to the C-3 position in protopanaxadiol (PPD)-type ginsenosides (EC 3.2.1.191) and the C-6 position in protopanaxatriol (PPT)-type ginsenosides (EC 3.2.1.194) except for the hydrolysis of gypenoside LXXV (GypLXXV). The enzyme converts gypenoside XVII (GypXVII) to GypLXXV by hydrolyzing the inner glucose linked to the C-3 position
-
-
?
additional information
?
-
-
recombinant wild-type beta-glucosidase from Clavibacter michiganensis specifically hydrolyzes the outer and inner glucose linked to the C-3 position in protopanaxadiol (PPD)-type ginsenosides (EC 3.2.1.191) and the C-6 position in protopanaxatriol (PPT)-type ginsenosides (EC 3.2.1.194) except for the hydrolysis of gypenoside LXXV (GypLXXV). The enzyme converts gypenoside XVII (GypXVII) to GypLXXV by hydrolyzing the inner glucose linked to the C-3 position
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
the enzyme from hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages
-
-
?
additional information
?
-
Ganoderma applanatum KACC 53688
-
the enzyme from hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages
-
-
?
additional information
?
-
-
the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages
-
-
?
additional information
?
-
Ganoderma lucidum KACC 42231
-
the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
Intrasporangium sp. GS603
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
beta-glucosidase Bgy2 cleaves the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. At optimal conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 are converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. No activity with 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 2-nitrophenyl-beta-D-galactopyranoside, and 4-nitrophenyl-alpha-L-arabinofuranoside. Product identification by mass spectrometry
-
-
?
additional information
?
-
beta-glucosidase Bgy2 cleaves the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. At optimal conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 are converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. No activity with 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 2-nitrophenyl-beta-D-galactopyranoside, and 4-nitrophenyl-alpha-L-arabinofuranoside. Product identification by mass spectrometry
-
-
?
additional information
?
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ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
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?
additional information
?
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ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
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?
additional information
?
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ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
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?
additional information
?
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Paecilomyces variotii 229-7
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ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
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?
additional information
?
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bioconversion of major ginsenosides to F2 during milk fermentation
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?
additional information
?
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recombinant His-tagged enzyme BGL hydrolyzes the outer glucose moiety of C-3 and C-20 positions of the major ginsenosides (Rb1 and Rd) and produces the minor ginsenoside F2: the pathway is Rb1 -> Rd -> F2. Furthermore, the enzyme transforms the minor ginsenoside Rg3 to Rh2: pathway Rg3 -> Rh2
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?
additional information
?
-
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bioconversion of major ginsenosides to F2 during milk fermentation
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-
?
additional information
?
-
-
recombinant His-tagged enzyme BGL hydrolyzes the outer glucose moiety of C-3 and C-20 positions of the major ginsenosides (Rb1 and Rd) and produces the minor ginsenoside F2: the pathway is Rb1 -> Rd -> F2. Furthermore, the enzyme transforms the minor ginsenoside Rg3 to Rh2: pathway Rg3 -> Rh2
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?
additional information
?
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the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages
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?
additional information
?
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Pleurotus ostreatus KACC 50356
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the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages
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?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages
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-
?
additional information
?
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Sanghuangporus baumii KACC 53719
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the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages
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?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
-
-
?
additional information
?
-
-
ginsenosidase type I enzymes simultaneously hydrolyze the sugars linked to C-20 and C-3 in the PPD-type ginsenosides and have broad hydrolysis patterns of A, B, C and D
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?
