Information on EC 3.2.1.193 - ginsenosidase type I

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The expected taxonomic range for this enzyme is: Aspergillus

EC NUMBER
COMMENTARY hide
3.2.1.193
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RECOMMENDED NAME
GeneOntology No.
ginsenosidase type I
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + H2O = a protopanaxadiol-type ginsenoside with no glycosidic modifications at position 3 + D-glucopyranose
show the reaction diagram
(2)
-
-
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a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + H2O = a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + D-glucopyranose
show the reaction diagram
a protopanaxadiol-type ginsenoside with two glycosyl residues at position 20 + H2O = a protopanaxadiol-type ginsenoside with a single glucosyl residue at position 20 + a monosaccharide
show the reaction diagram
(3)
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ginsenoside degradation I
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ginsenoside degradation III
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ginsenoside metabolism
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SYSTEMATIC NAME
IUBMB Comments
ginsenoside glucohydrolase
Ginsenosidase type I is slightly activated by Mg2+ or Ca2+ [1]. The enzyme hydrolyses the 3-O-beta-D-(1->2)-glucosidic bond, the 3-O-beta-D-glucopyranosyl bond and the 20-O-beta-D-(1->6)-glycosidic bond of protopanaxadiol-type ginsenosides. It usually leaves a single glucosyl residue attached at position 20 and one or no glucosyl residues at position 3. Starting with a ginsenoside that is glycosylated at both positions (e.g. ginsenoside Rb1, Rb2, Rb3, Rc or Rd), the most common products are ginsenoside F2 and ginsenoside C-K, with low amounts of ginsenoside Rh2.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ginsenoside Rb1 + H2O
ginsenoside F2 + 2 D-glucose
show the reaction diagram
ginsenoside Rb2 + H2O
ginsenoside F2 + D-glucose + L-arabinopyranose
show the reaction diagram
ginsenoside Rb3 + H2O
ginsenoside F2 + D-glucose + D-xylose
show the reaction diagram
ginsenoside Rc + H2O
ginsenoside F2 + D-glucose + L-arabinofuranose
show the reaction diagram
ginsenoside Rd + H2O
ginsenoside + D-glucose
show the reaction diagram
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ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside
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-
?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
50-100 mM, slight activation
Mg2+
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50-100 mM, slight activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.4
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40°C, pH not specified in the publication, substrate: ginsenoside Rg3
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 8
-
pH 4.0: about 80% of maximal activity, pH 8.0: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 70
-
20°C: about 60% of maximal activity, 70°C: about 60% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
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x * 80000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 7
-
stable
722033
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 40
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48 h, stable
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE