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3.2.1.193: ginsenosidase type I

This is an abbreviated version!
For detailed information about ginsenosidase type I, go to the full flat file.

Word Map on EC 3.2.1.193

Reaction

a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3
+
H2O
=
a protopanaxadiol-type ginsenoside with no glycosidic modifications at position 3
+
D-glucopyranose

Synonyms

beta-glucosidase, BG-1, Bgl, BglPm, bgy2, ginsenosidase type I, ginsenosidase type-I, ginsenoside Rc-hydrolyzing beta-glucosidase, ginsenoside-hydrolyzing beta-glucosidase, LVISKB_0241, More

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.193 ginsenosidase type I

Purification

Purification on EC 3.2.1.193 - ginsenosidase type I

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 33.8fold from mycelium by ammonium sulfate fractionation, anion exchange chromatographydialysis, lyophilization, and gel filtration
-
native enzyme from mycelium by anion exchange chromatography to homogeneity
-
recombinant C-terminally His6-tagged enzyme from Lactococcus lactis strain NZ9000 by nickel affinity chromatography
-