3.2.1.167: baicalin-beta-D-glucuronidase

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For detailed information about baicalin-beta-D-glucuronidase, go to the full flat file.

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Synonyms

baicalin-beta-D-glucuronidase, baicalinase, beta-glucuronidase, EC 3.2.1.31, GUS, HP-GUS, LcGUS30, sGUS

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.167 baicalin-beta-D-glucuronidase

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Results	in table
1	Activating Compound
2	AA Sequence
3	Application
3	Cloned(Commentary)
2	Expression
6	General Information
2	General Stability
13	Inhibitors
10	kcat/KM [mM/s]
15	KM Value [mM]
1	Localization
3	Metals/Ions
10	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
17	Organism
3	Pathway
11	pH Optimum
4	pH Range
9	pH Stability
4	pI Value
5	Protein Variants
7	Purification (Commentary)
1	Reaction
16	Reference
11	Source Tissue
7	Specific Activity [micromol/min/mg]
9	Storage Stability
48	Substrates and Products (Substrate)
10	Subunits
23	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
3	Temperature Range [°C]
8	Temperature Stability [°C]
9	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.2.1.167 - baicalin-beta-D-glucuronidase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 10 mM NaH2PO4/Na2HPO4 buffer, 1 mM mercaptoethanol, pH 7.0, 50% glycerol, 2 months, 10-20% loss of activity

Scutellaria baicalensis

-

657119

4°C, 10 mM NaH2PO4/Na2HPO4 buffer, 1 mM mercaptoethanol, pH 7.0, 50% glycerol, 2 weeks, complete loss of activity

Scutellaria baicalensis

-

657119

4°C, 30 mM Tris-HCl pH 7.0, 11 days, 26% loss of activity

Escherichia coli

-

712143

4°C, 30 mM Tris-HCl pH 7.0, 5 days, 19% loss of activity

Escherichia coli

-

712143

4°C, free enzyme in 30 mM Tris–HCl (pH 7.0), 11 days, about 25% loss of activity

Escherichia coli

-

709548

4°C, free enzyme in 30 mM Tris–HCl (pH 7.0), 26 days, 96% loss of activity

Escherichia coli

-

709548

4°C, free enzyme in 30 mM Tris–HCl (pH 7.0), 5 days, about 20% loss of activity

Escherichia coli

-

709548

4°C, GUS encapsulated in biomimetic alginate/protamine/silica capsules in 30 mM Tris–HCl (pH 7.0), 11 days, no loss of activity

Escherichia coli

-

709548

4°C, GUS encapsulated in biomimetic alginate/protamine/silica capsules in 30 mM Tris–HCl (pH 7.0), 26 days, 10% loss of activity

Escherichia coli

-

709548