Literature summary for 3.2.1.167 extracted from

Jiang, Z.; Zhang, Y.; Li, J.; Jiang, W.; Yang, D.; Wu, H.
Encapsulation of beta-glucuronidase in biomimetic alginate capsules for bioconversion of baicalin to baicalein (2007), Ind. Eng. Chem. Res., 46, 1883-1890.

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General Stability

General Stability	Organism
the calcium alginate-encapsulated GUS retains up to 88% of its free-form activity with an encapsulation efficiency of 77%. Conversion of baicalin by free and calcium alginate-encapsulated GUS results in the baicalein productivities of 80% and 65%, respectively. The calcium alginate-encapsulated GUS shows no appreciable loss in activity after four repeated cycles, and 90% of its initial activity remains after 26-day storage at 4°C	Escherichia coli

Organism

the calcium alginate-encapsulated GUS retains up to 88% of its free-form activity with an encapsulation efficiency of 77%. Conversion of baicalin by free and calcium alginate-encapsulated GUS results in the baicalein productivities of 80% and 65%, respectively. The calcium alginate-encapsulated GUS shows no appreciable loss in activity after four repeated cycles, and 90% of its initial activity remains after 26-day storage at 4°C

Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	the free enzyme activity decreases rapidly and is even totally lost when the Ca2+ concentration is higher than 5 mM	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.19	-	5,6,7-trihydroxyflavone-7-beta-D-glucuronide	free enzyme, at pH 7.0 and 37°C	Escherichia coli
0.36	-	5,6,7-trihydroxyflavone-7-beta-D-glucuronide	calcium alginate-encapsulated enzyme, at pH 7.0 and 37°C	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Escherichia coli	5764	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
290000	-	gel filtration	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Storage Stability

Storage Stability	Organism
4°C, 30 mM Tris-HCl pH 7.0, 11 days, 26% loss of activity	Escherichia coli
4°C, 30 mM Tris-HCl pH 7.0, 5 days, 19% loss of activity	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,6,7-trihydroxyflavone-7-beta-D-glucuronide + H2O	-	Escherichia coli	5,6,7-trihydroxyflavone + D-glucuronate	-	?

Synonyms

Synonyms	Comment	Organism
beta-glucuronidase	-	Escherichia coli
EC 3.2.1.31	-	Escherichia coli
GUS	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	70	the free enzyme shows about 45% relative activity at 30°C, about 65% relative activity at 40°C, about 80% relative activity at 50°C, 100% activity at 60°C, and about 75% relative activity at 70°C (at pH 7.0, reaction time 40 min)	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Escherichia coli

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	8	the free enzyme shows no activity at pH 4.0, about 85% relative activity at pH 5.5 and pH 6.5, 100% activity at pH 7.0, about 60% relative activity at pH 7.5, and no activity at pH 8.0 (at 37°C, reaction time 60 min)	Escherichia coli

pI Value

Organism	Comment	pI Value Maximum	pI Value
Escherichia coli	isoelectric focusing	-	4.8