3.2.1.164: galactan endo-1,6-beta-galactosidase

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For detailed information about galactan endo-1,6-beta-galactosidase, go to the full flat file.

Reaction

Endohydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan proteins and (1->3):(1->6)-beta-galactans to yield galactose and (1->6)-beta-galactobiose as the final products =

Synonyms

6GAL, beta-(1,6)-galactanase, beta-1,6-galactanase, beta-D-galactanase, ebg, endo-beta-(1,6)-D-galactanase, endo-beta-(1-6)-D-galactanase, endo-beta-(1-6)-galactanase, endo-beta-(1->6)-D-galactanase, endo-beta-(1->6)-galactanase, endo-beta-1,6-D-galactanase, endo-beta-1,6-galactanase, GAL1, More, Tv6GAL, type II arabinogalactan-degrading enzyme

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.164 galactan endo-1,6-beta-galactosidase

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Results	in table
21	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
1	Expression
3	General Information
7	Inhibitors
2	KM Value [mM]
2	Localization
1	Metals/Ions
13	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
9	Organism
4	pH Optimum
1	pH Range
2	pH Stability
1	Posttranslational Modification
6	Purification (Commentary)
3	Reaction
9	Reference
5	Source Tissue
5	Specific Activity [micromol/min/mg]
16	Substrates and Products (Substrate)
7	Subunits
21	Synonyms
4	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Temperature Stability [°C]

Purification

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Purification on EC 3.2.1.164 - galactan endo-1,6-beta-galactosidase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

CM-32 column chromatography, DEAE-Sepharose column chromatography and Sephadex G-100 gel filtration

Neurospora crassa

Q7S2S4

714656

native enzyme 26.2fold to homogeneity by several chromatographic steps, overview

Aspergillus niger

-

671192

native enzyme form a commercial cellulase preparation, 57fold to homogeneity by ammonium sulfate fractionation, two steps of ion exchange chromatography, and gel filtration

Trichoderma viride

-

672925

native enzyme from commercial pectinase preparation by anion and cation exchange chromatography, ultrafiltration, and gel filtration

Aspergillus niger

-

655166

recombinant His6-tagged enzyme from Escherichia coli strain Bl21 by two steps of metal chelating chromatography, the thioredoxin tag is cleaved off

Trichoderma viride

Q76FP5

671821

recombinant maltose-binding-protein fusion enzyme from Escherichia coli strain DH5alpha, by cation exchange and amylose affinity chromatography, followed by hydrophobic interaction chromatography, native enzyme 88fold from strain S12 culture filtrate by anion exchange and hydrophobic interaction chromatography, followed by cation exchange chromatography, dialysis and gel filtration

Fusarium oxysporum

-

671490