3.2.1.164: galactan endo-1,6-beta-galactosidase
This is an abbreviated version!
For detailed information about galactan endo-1,6-beta-galactosidase, go to the full flat file.
Word Map on EC 3.2.1.164
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3.2.1.164
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galactose
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oligosaccharides
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helicase
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larch
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wood
- 3.2.1.164
- galactose
- oligosaccharides
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helicase
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larch
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wood
Reaction
Endohydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan proteins and (1->3):(1->6)-beta-galactans to yield galactose and (1->6)-beta-galactobiose as the final products =
Synonyms
6GAL, beta-(1,6)-galactanase, beta-1,6-galactanase, beta-D-galactanase, ebg, endo-beta-(1,6)-D-galactanase, endo-beta-(1-6)-D-galactanase, endo-beta-(1-6)-galactanase, endo-beta-(1->6)-D-galactanase, endo-beta-(1->6)-galactanase, endo-beta-1,6-D-galactanase, endo-beta-1,6-galactanase, GAL1, More, Tv6GAL, type II arabinogalactan-degrading enzyme
ECTree
3.2.1.164 galactan endo-1,6-beta-galactosidaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.2.1.164 - galactan endo-1,6-beta-galactosidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
arabinogalactan-protein + H2O
beta-GlcA-(1->6)-Gal + 4-methyl-beta-GlcA-(1->6)-Gal
the enzyme prefers alpha-L arabinofuranosidase-treated arabinogalactan-proteins from radish roots (151% activity) and radish leaves (87% activity) compared to native ones from radish roots (36% activity) and leaves (28% activity)
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?
beta-(1->6)-galactotriose + H2O
beta-(1->6)-galactobiose + galactose
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?
beta-galactan + H2O
?
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galactan from Prototheca zopfii, which has a high proportion of beta-1,6-linked galactosyl residues, the extent of hydrolysis of the carbohydrate portion of a radish root arabinogalactan-protein AGP increases when alpha-L-arabinofuranosyl residues attached to beta-1,6-linked galactosyl side chains of the AGP are removed in advance, the enzyme releases galactose, beta-1,6-galactobiose, and 4-O-methyl-beta-glucuronosyl-1,6-galactose as major hydrolysis products when allowed to act exhaustively on the modified AGP, overview
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?
desarabinosylated grape 3,6-galactan-protein + H2O
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the enzyme liberates beta-1,6-D-galactobiose and galactose
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?
arabinogalactan + H2O
oligo-beta-(1->6)-galactans + beta-(1->6)-D-galactobiose
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?
arabinogalactan + H2O
oligo-beta-(1->6)-galactans + beta-(1->6)-D-galactobiose
plant type II arabinogalactans consisting of beta-(1,3)- and beta-(1,6)-galactan side chains connected to each other by (1,3)(1,6)-linked branch points. The O-3 and O-6 positions are substituted with terminal arabinosyl residues
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?
type II arabinogalactans + H2O
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the enzyme preferentially debranches beta-1,6-galactobiose from the substrate, type II arabinogalactans from Western larch
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?
type II arabinogalactans + H2O
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the enzyme preferentially debranches beta-1,6-galactobiose from the substrate, type II arabinogalactans from Western larch
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?
additional information
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activity with galactans from different sources, overview
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?
additional information
?
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the beta-1,6-galactanase liberates galactose and 1,6-galactobiose from acid-treated larchwood and Norway spruce arabinogalactans
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?
additional information
?
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the enzyme does not cleave beta-D-1,3-galactosidic bonds
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?
additional information
?
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the enzyme fails to act on gum arabic, beta-(1->6)-galactobiose beza-(1->4)-galactan from lupin, beta-(1->3)(1->4)-glucan from barley, beta-(1->3)(1->6)-glucan from Laminaria digitata, beta-(1->6)-glucan from Umbilicaria papullosa, carboxymethyl-curdlan (beta-(1->3)-glucan), carboxymethyl-cellulose (beta-(1->4)-glucan), galactomannan from guar, galactomannan from locust bean, beta-(1->4)-xylan from birchwood, arabino-beta-(1->4)-xylan from wheat flour, arabinan (alpha-(1->3)(1->5)-arabinan), and debranched arabinan (alpha-(1->5)-arabinan)
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?
additional information
?
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substrate preparations and specificity, the endo-galactanase hydrolyzes specifically beta-1,6-galactooligosaccharides with a degree of polymerization higher than 3 and their acidic derivatives with 4-O-methyl-glucosyluronic or glucosyluronic groups at the nonreducing terminals, oveview
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