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Literature summary for 3.2.1.164 extracted from
- Molecular cloning and expression in Escherichia coli of a Trichoderma viride endo-beta-(1-6)-galactanase gene (2004), Biochem. J., 377, 749-755.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and maino acid sequence determination and analysis, sequence comparison, expression in Escherichia coli strain BL21 as His6-tagged enzyme fused to thioredoxin | Trichoderma viride |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50488 | - |
x * 50488, sequence calculation, x * 64000, recombinant enzyme, SDS-PAGE, x * 50687, detagged recombinant enzyme, mass spectrometry, x * 68296, recombinant His6-tagged thioredoxin fusion enzyme, sequence calculation | Trichoderma viride |
| 50687 | - |
x * 50488, sequence calculation, x * 64000, recombinant enzyme, SDS-PAGE, x * 50687, detagged recombinant enzyme, mass spectrometry, x * 68296, recombinant His6-tagged thioredoxin fusion enzyme, sequence calculation | Trichoderma viride |
| 64000 | - |
x * 50488, sequence calculation, x * 64000, recombinant enzyme, SDS-PAGE, x * 50687, detagged recombinant enzyme, mass spectrometry, x * 68296, recombinant His6-tagged thioredoxin fusion enzyme, sequence calculation | Trichoderma viride |
| 68296 | - |
x * 50488, sequence calculation, x * 64000, recombinant enzyme, SDS-PAGE, x * 50687, detagged recombinant enzyme, mass spectrometry, x * 68296, recombinant His6-tagged thioredoxin fusion enzyme, sequence calculation | Trichoderma viride |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma viride | Q76FP5 | IFO 31 137 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain Bl21 by two steps of metal chelating chromatography, the thioredoxin tag is cleaved off | Trichoderma viride |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Endohydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan proteins and (1->3):(1->6)-beta-galactans to yield galactose and (1->6)-beta-galactobiose as the final products | mode of action, overview | Trichoderma viride |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity, overview | Trichoderma viride | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50488, sequence calculation, x * 64000, recombinant enzyme, SDS-PAGE, x * 50687, detagged recombinant enzyme, mass spectrometry, x * 68296, recombinant His6-tagged thioredoxin fusion enzyme, sequence calculation | Trichoderma viride |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-(1-6)-galactanase | - |
Trichoderma viride |
| Tv6GAL | - |
Trichoderma viride |
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