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3.2.1.162: lambda-carrageenase

This is an abbreviated version!
For detailed information about lambda-carrageenase, go to the full flat file.

Word Map on EC 3.2.1.162

Reaction

Endohydrolysis of (1->4)-beta-linkages in the backbone of lambda-carrageenan, resulting in the tetrasaccharide alpha-D-Gal-p2,6S2-(1->3)-beta-D-Gal-p2S-(1->4)-alpha-D-Gal-p2,6S2-(1->3)-D-Gal-p2-S =

Synonyms

Cga-L50, CglA, CglA hydrolase, CglCL19, Cglp, CglPc, delta-carrageenase, endo-beta-1,4-carrageenose 2,6,2'-trisulfate-hydrolase, endo-type delta-carrageenase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.162 lambda-carrageenase

Cloned

Cloned on EC 3.2.1.162 - lambda-carrageenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as His-tag fusion protein in Escherichia coli, formation of inclusion bodies that are solved in 8 M urea
expression in Escherichia coli
expression in Escherichia coli as insoluble inclusion bodies. These inclusion bodies are purified and solubilized in 8 M urea
gene cglA, phylogenetic analysis, overview
subcloning in Escherichia coli strain DH5alpha, recombinant expression of a chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain in Escherichia coli strain BL21(DE3)
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