Application | Comment | Organism |
---|---|---|
agriculture | the assemblies of advancement of active enzyme complexes, i.e. of kappa-carrageenase CgkA and delta-carrageenase CglA, will facilitate the commercial production of useful products from red algae biomass whichrepresents inexpensive and sustainable feed-stocks | Pseudoalteromonas carrageenovora |
Cloned (Comment) | Organism |
---|---|
subcloning in Escherichia coli strain DH5alpha, recombinant expression of a chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain in Escherichia coli strain BL21(DE3) | Pseudoalteromonas carrageenovora |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain, miniCbpA, cCgkA and cCglA assemble into a complex, the dockerin-fused enzymes on the scaffoldin have synergistic activity in the degradationof carrageenan showing enhancement of activity by carrageenolytic complex 3.1fold higher compared with the corresponding enzymes alone | Pseudoalteromonas carrageenovora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
109000 | - |
gel filtration | Pseudoalteromonas carrageenovora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas carrageenovora | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant chimeric protein cCgkA and cCglA containing the catalytic domain of kappa-carrageenase CgkA and delta-carrageenase CglA fused with a dockerin domain from Escherichia coli strain BL21(DE3) by cellulose affinity chromatography using carbohydrate binding module in scaffoldin miniCbpA as a tag | Pseudoalteromonas carrageenovora |
Subunits | Comment | Organism |
---|---|---|
? | x * 109000, His-tagged enzyme, SDS-PAGE | Pseudoalteromonas carrageenovora |
Synonyms | Comment | Organism |
---|---|---|
CglA | - |
Pseudoalteromonas carrageenovora |
delta-carrageenase | - |
Pseudoalteromonas carrageenovora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Pseudoalteromonas carrageenovora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudoalteromonas carrageenovora |