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3.2.1.158: alpha-agarase

This is an abbreviated version!
For detailed information about alpha-agarase, go to the full flat file.

Word Map on EC 3.2.1.158

Reaction

((1-4)-3,6-anhydro-alpha-L-galactopyranosyl-(1-3)-beta-D-galactopyranose)n
+
H2O
=
((1-4)-3,6-anhydro-alpha-L-galactopyranosyl-(1-3)-beta-D-galactopyranose)4
+
((1-4)-3,6-anhydro-alpha-L-galactopyranosyl-(1-3)-beta-D-galactopyranose)n-4

Synonyms

AgaA, AgaA33, AgaD, agaraseA33, alpha-agarase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.158 alpha-agarase

Purification

Purification on EC 3.2.1.158 - alpha-agarase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hydroxyapatite chromatography and gel filtration
isolated from the sediment of Noma point, Japan, at a depth of 230m, purified by DEAE-Toyopearl 650M column, ultracentrifugation and gel filtration
-
Ni-Sepharose column chromatography and Superdex S200 gel filtration