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3.2.1.158: alpha-agarase

This is an abbreviated version!
For detailed information about alpha-agarase, go to the full flat file.

Word Map on EC 3.2.1.158

Reaction

((1-4)-3,6-anhydro-alpha-L-galactopyranosyl-(1-3)-beta-D-galactopyranose)n
+
H2O
=
((1-4)-3,6-anhydro-alpha-L-galactopyranosyl-(1-3)-beta-D-galactopyranose)4
+
((1-4)-3,6-anhydro-alpha-L-galactopyranosyl-(1-3)-beta-D-galactopyranose)n-4

Synonyms

AgaA, AgaA33, AgaD, agaraseA33, alpha-agarase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.158 alpha-agarase

Cloned

Cloned on EC 3.2.1.158 - alpha-agarase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
agaA gene isolated from a genomic library of Alteromonas agarilytica, alpha-agarase precursor of 1429 amino acid residues, expressed in Escherichia coli, novel catalytic domain defines a new GH family
expressed in Bacillus subtilis
expressed in Escherichia coli AD494(DE3) cells
expressed in Escherichia coli HB101, open reading frame of 1463 amino acid residues of the agaA33 gene determined, for extracellular production of recombinant alpha-agarase AgaA33 the Bacillus subtilis strain ISW1214 serves as host and plasmid pEXBS as expression vector
expressed in Saccharomyces cerevisiae strains SEY2102 and FY833 and in Pichia pastoris strain GS115
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