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Literature summary for 3.2.1.158 extracted from

  • Ohta, Y.; Hatada, Y.; Miyazaki, M.; Nogi, Y.; Ito, S.; Horikoshi, K.
    Purification and characterization of a novel alpha-agarase from a Thalassomonas sp. (2005), Curr. Microbiol., 50, 212-216.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Thalassomonas sp.

General Stability

General Stability Organism
Ca2+ stabilizes enzyme Thalassomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular using Bacillus subtilis as the host Thalassomonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, about 15% residual activity in absence of Ca2+ Thalassomonas sp.
Ca2+ stabilizes enzyme Thalassomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
gel filtration Thalassomonas sp.
85000
-
SDS-PAGE and gel filtration Thalassomonas sp.

Organism

Organism UniProt Comment Textmining
Thalassomonas sp.
-
-
-
Thalassomonas sp. JAMB-A33
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thalassomonas sp.
isolated from the sediment of Noma point, Japan, at a depth of 230m, purified by DEAE-Toyopearl 650M column, ultracentrifugation and gel filtration Thalassomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information isolated from the sediment of Noma point, Japan, at a depth of 230m Thalassomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.7
-
-
Thalassomonas sp.
40.7
-
pH 8.5, 45°C Thalassomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarohexaose + H2O
-
Thalassomonas sp. agarotetraose + agarobiose
-
?
agarohexaose + H2O
-
Thalassomonas sp. JAMB-A33 agarotetraose + agarobiose
-
?
agarohexaose + H2O
-
Thalassomonas sp. ?
-
?
agarose + H2O
-
Thalassomonas sp. agarotetraose + ?
-
?
agarose + H2O
-
Thalassomonas sp. JAMB-A33 agarotetraose + ?
-
?
additional information no substrate: kappa-, or lambda-carrageenans, p-nitrophenyl-galactopyranosides Thalassomonas sp. ?
-
?
additional information no substrate: kappa-, or lambda-carrageenans, p-nitrophenyl-galactopyranosides Thalassomonas sp. JAMB-A33 ?
-
?
neoagarohexaose + H2O
-
Thalassomonas sp. agarotetraose
-
?
neoagarohexaose + H2O
-
Thalassomonas sp. JAMB-A33 agarotetraose
-
?
neoagarohexaose + H2O
-
Thalassomonas sp. ?
-
?
porphyran + H2O
-
Thalassomonas sp. ?
-
?
porphyran + H2O at 95% of the rate with agarose Thalassomonas sp. ?
-
?
porphyran + H2O
-
Thalassomonas sp. JAMB-A33 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 85000, SDS-PAGE Thalassomonas sp.

Synonyms

Synonyms Comment Organism
agaraseA33
-
Thalassomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Thalassomonas sp.
45
-
50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer, pH 8.5, containing 5 mM CaCl2 Thalassomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable for at least 30 min Thalassomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Thalassomonas sp.
8.5
-
25°C, 30 min, different buffers Thalassomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.5
-
Thalassomonas sp.
6 11
-
Thalassomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11
-
Thalassomonas sp.