Cloned (Comment) | Organism |
---|---|
- |
Thalassomonas sp. |
General Stability | Organism |
---|---|
Ca2+ stabilizes enzyme | Thalassomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | using Bacillus subtilis as the host | Thalassomonas sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, about 15% residual activity in absence of Ca2+ | Thalassomonas sp. | |
Ca2+ | stabilizes enzyme | Thalassomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
gel filtration | Thalassomonas sp. |
85000 | - |
SDS-PAGE and gel filtration | Thalassomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thalassomonas sp. | - |
- |
- |
Thalassomonas sp. JAMB-A33 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thalassomonas sp. |
isolated from the sediment of Noma point, Japan, at a depth of 230m, purified by DEAE-Toyopearl 650M column, ultracentrifugation and gel filtration | Thalassomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | isolated from the sediment of Noma point, Japan, at a depth of 230m | Thalassomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40.7 | - |
- |
Thalassomonas sp. |
40.7 | - |
pH 8.5, 45°C | Thalassomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarohexaose + H2O | - |
Thalassomonas sp. | agarotetraose + agarobiose | - |
? | |
agarohexaose + H2O | - |
Thalassomonas sp. JAMB-A33 | agarotetraose + agarobiose | - |
? | |
agarohexaose + H2O | - |
Thalassomonas sp. | ? | - |
? | |
agarose + H2O | - |
Thalassomonas sp. | agarotetraose + ? | - |
? | |
agarose + H2O | - |
Thalassomonas sp. JAMB-A33 | agarotetraose + ? | - |
? | |
additional information | no substrate: kappa-, or lambda-carrageenans, p-nitrophenyl-galactopyranosides | Thalassomonas sp. | ? | - |
? | |
additional information | no substrate: kappa-, or lambda-carrageenans, p-nitrophenyl-galactopyranosides | Thalassomonas sp. JAMB-A33 | ? | - |
? | |
neoagarohexaose + H2O | - |
Thalassomonas sp. | agarotetraose | - |
? | |
neoagarohexaose + H2O | - |
Thalassomonas sp. JAMB-A33 | agarotetraose | - |
? | |
neoagarohexaose + H2O | - |
Thalassomonas sp. | ? | - |
? | |
porphyran + H2O | - |
Thalassomonas sp. | ? | - |
? | |
porphyran + H2O | at 95% of the rate with agarose | Thalassomonas sp. | ? | - |
? | |
porphyran + H2O | - |
Thalassomonas sp. JAMB-A33 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 85000, SDS-PAGE | Thalassomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
agaraseA33 | - |
Thalassomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Thalassomonas sp. |
45 | - |
50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer, pH 8.5, containing 5 mM CaCl2 | Thalassomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable for at least 30 min | Thalassomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Thalassomonas sp. |
8.5 | - |
25°C, 30 min, different buffers | Thalassomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | - |
Thalassomonas sp. |
6 | 11 | - |
Thalassomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | - |
Thalassomonas sp. |