3.2.1.102: blood-group-substance endo-1,4-beta-galactosidase

This is an abbreviated version, for detailed information about blood-group-substance endo-1,4-beta-galactosidase, go to the full flat file.

Reaction

Endohydrolysis of (1->4)-beta-D-galactosidic linkages in blood group A and B substances =

Synonyms

ABase, blood group A- and B-cleaving endo-beta-galactosidase, blood group A/B antigen-specific endo-beta-galactosidase, blood group antigen-cleaving endo-beta-galactosidase, blood group glycotope-cleaving endo-beta-galactosidase, E-ABase, EABase, endo-beta-galactosidase, endo-beta-galactosidase C, endo-beta-galactosidase DII, EndoGalC, galactosidase, endo-beta-, GH98CBM51, inverting endo-beta-galactosidase, More, Sp3GH98, Sp4GH98

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.102 blood-group-substance endo-1,4-beta-galactosidase

Purification

Purification on EC 3.2.1.102 - blood-group-substance endo-1,4-beta-galactosidase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and MonoQ column chromatography
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immobilized metal affinity chromatography
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native enzyme from strain ATCC 10543, by gel filtration, ion exchange, adsorption, and concanavalin A affinity chromatography, and another step of ion exchange chromatography, 2630fold to homogeneity, recombinant His-tagged enzyme by nickel affinity chromatography
Ni-NTA column chromatography and Sephacryl S-200 gel filtration
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nickel-nitrilotriacetic acid column
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