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Literature summary for 3.2.1.102 extracted from
- Mechanistic analysis of the blood group antigen-cleaving endo-beta-galactosidase from Clostridium perfringens (2009), Biochemistry, 48, 8396-8404.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Clostridium perfringens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D429A | the mutants shows reduced activity compared to the wild type enzyme | Clostridium perfringens |
| D453A | the mutation results in complete loss of activity | Clostridium perfringens |
| E354A | the activity of the E354A mutant with nonactivated natural substrates is 1100fold lower than that of the wild type enzyme, while its activity is only 10fold lower when assayed with 2,4-dinitrophenyl-beta-A-trisaccharide | Clostridium perfringens |
| E467A | the mutants shows slightly reduced activity compared to the wild type enzyme | Clostridium perfringens |
| E506A | the mutation results in complete loss of activity | Clostridium perfringens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.064 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | wild type enzyme, at pH 6.0 and 37°C | Clostridium perfringens |  |
| 0.31 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | mutant enzyme E354A, at pH 6.0 and 37°C | Clostridium perfringens | |
| 0.59 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | mutant enzyme E467A, at pH 6.0 and 37°C | Clostridium perfringens | |
| 0.61 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | mutant enzyme D429A, at pH 6.0 and 37°C | Clostridium perfringens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
SDS-PAGE | Clostridium perfringens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| blood group A glycoconjugate + H2O | Clostridium perfringens | - |
? | - |
? | |
| blood group B glycoconjugate + H2O | Clostridium perfringens | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
strain ATCC 10543 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography and MonoQ column chromatography | Clostridium perfringens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside + H2O | - |
Clostridium perfringens | ? | - |
? | |
| blood group A glycoconjugate + H2O | - |
Clostridium perfringens | ? | - |
? | |
| blood group B glycoconjugate + H2O | - |
Clostridium perfringens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| blood group antigen-cleaving endo-beta-galactosidase | - |
Clostridium perfringens |
| EABase | - |
Clostridium perfringens |
| inverting endo-beta-galactosidase | - |
Clostridium perfringens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | mutant enzyme E354A, at pH 6.0 and 37°C | Clostridium perfringens | |
| 1.35 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | mutant enzyme D429A, at pH 6.0 and 37°C | Clostridium perfringens | |
| 1.55 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | mutant enzyme E467A, at pH 6.0 and 37°C | Clostridium perfringens | |
| 1.75 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | wild type enzyme, at pH 6.0 and 37°C | Clostridium perfringens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.33 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | mutant enzyme E354A, at pH 6.0 and 37°C | Clostridium perfringens | |
| 21.67 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | mutant enzyme D429A, at pH 6.0 and 37°C | Clostridium perfringens | |
| 26.67 | - |
2,4-dinitrophenyl 2-acetamido-2-deoxy-D-galactopyranosyl-(1,3)-[alpha-L-fucopyranosyl-(1,2)]-beta-D-galactopyranoside | mutant enzyme E467A, at pH 6.0 and 37°C | Clostridium perfringens | |
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