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3.1.6.8: cerebroside-sulfatase

This is an abbreviated version!
For detailed information about cerebroside-sulfatase, go to the full flat file.

Word Map on EC 3.1.6.8

Reaction

a cerebroside 3-sulfate
+
H2O
=
a cerebroside
 +
sulfate

Synonyms

ARS, ARSA, arylsulfatase A, arylsulfatase E, arylsulfatase-A, AS-A, ASA, cerebroside sulfatase, cerebroside sulfate sulfatase, Cerebroside-sulfatase, sulfatase, cerebroside

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.6 Sulfuric-ester hydrolases
                3.1.6.8 cerebroside-sulfatase

Cloned

Cloned on EC 3.1.6.8 - cerebroside-sulfatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpreesion with mannose 6-phosphate receptor
-
coexpression and secretion of misfolded, enzymatically inactive enzyme with the wild-type enzyme, whose activity is diminished upon coexpression with misfolded ASA polypeptides, in BHK cells
-
exprerssion in multipotent mesenchymal stromal cells
expressed in baby hamster kidney cells and CHO cells
-
expressed in CHO cells
expression in arylsulfatase A-deficient mice, HEK-293 cells, HeLa cells and COS-7 cells
-
expression in BHK cells
expression of wild-type and mutant enzymes in CHO-K1 cells
HSPC cells transduced with increasing doses of lentiviral vectors expressing ARSA under the control of the human PGK promoter (PGK.ARSA.LV). Transgenic mice generated with PGK.ARSA.LV overexpressing the ARSA enzyme
-
human arylsulfatase A tagged with hemagglutinin or GFP cloned in a lentiviral vector, expressed in HeLa cells and mice
-
mutation expressed from allele are examined in heterologous BHK cells
-
overexpression in CHO-K1 cells
plasmids containing the wild-type and mutant cDNAs are transiently transfected into BHK cells
-
recombinant expression in CHO, BHK and HT-1080 cells. High variability of the high-mannose-type N-glycans, which prevail at all glycosylation sites, depending on the culture conditions and the cell line expressing the enzyme, overview
-
recombinant expression of myc-His- or GFP-tagged ArsA, the recombinant protein is secreted into the serum-free medium
the ARSA gene is a small gene located on chromosome 22q13 that spans 8 exons and encodes a 507-aminoacid peptide, it is transcribed into three mRNA species, a major one of 2.1 kb and two less abundant species of 3.7 and 4.8 kb. DNA and amino acid sequence determination and analysis of natural mutants. Recombinant expression in HeLa cells using lentiviral vectors, containing mutated ARSA alleles, the vectors stably integrate into the host genome. expression of mutated alleles in murine ARSA-/- fibroblasts
transient expression experiments on COS7 cells transfected with enzyme cDNAs carrying the mutations separately and in the combination found in the patient's alleles
transient expression experiments on COS7 cells transfected with enzyme cDNAs carrying the mutations separately and in the combination found in the patients alleles
wild-type and mutant enzymes transiently transfected into BHK cells
-