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Literature summary for 3.1.6.8 extracted from

  • Matzner, U.; Breiden, B.; Schwarzmann, G.; Yaghootfam, A.; Fluharty, A.L.; Hasilik, A.; Sandhoff, K.; Gieselmann, V.
    Saposin B-dependent reconstitution of arylsulfatase a activity in vitro and in cell culture models of metachromatic leukodystrophy (2009), J. Biol. Chem., 284, 9372-9381.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.8

Activating Compound

Activating Compound Comment Organism Structure
bis(monoacylglycero) phosphate at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.4 Homo sapiens
dolichol at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 2.7 Homo sapiens
additional information phosphatidylinositol and phosphatidylserine have no effect on the rate of sulfatide hydrolysis Homo sapiens
phosphatidic acid at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.1 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in baby hamster kidney cells and CHO cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ CaCl2 reduces the reaction rate in the low millimolar range Homo sapiens
Mg2+ MgCl2 reduces the reaction rate in the low millimolar range Homo sapiens
Mn2+ MnCl2 reduces the reaction rate in the low millimolar range Homo sapiens
Na+ enzymatic activity is inhibited at NaCl concentrations exceeding 20 mM, at 50 and 200 mM the rate of sulfatide hydrolysis is diminished by about 50 and 75%, respectively Homo sapiens
phosphate competitive inhibitor Homo sapiens
sulfite competitive inhibitor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrocatechol sulfate + H2O
-
 Homo sapiens 4-nitrocatechol + sulfate
-
 ?
4-nitrophenyl sulfate + H2O
-
 Homo sapiens 4-nitrophenol + sulfate
-
 ?
cerebroside 3-sulfate + H2O the reaction requires saposin B, a non-enzymatic proteinaceous cofactor which presents sulfatide to the catalytic site of arylsulfatase A, desulfation is negligible when Sap B is substituted by Sap A, C, or D Homo sapiens cerebroside + sulfate
-
 ?

Synonyms

Synonyms Comment Organism
arylsulfatase A
-
 Homo sapiens
ASA
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3
-
 narrow pH optimum around pH 4.3 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3.75 4.75 desulfation activity is less than half-maximum at pH values below pH 3.75 and above pH 4.75 Homo sapiens