3.1.6.8: cerebroside-sulfatase

This is an abbreviated version!
For detailed information about cerebroside-sulfatase, go to the full flat file.

Word Map on EC 3.1.6.8

3.1.6.8

metachromatic

leukodystrophy

sulfatide

medicine

lysosomal

asa

demyelinate

sural

pseudodeficiency

nitrocatechol

sulfatases

molecular biology

Reaction

a cerebroside 3-sulfate

+

H2O

=

a cerebroside

+

sulfate

Synonyms

ARS, ARSA, arylsulfatase A, arylsulfatase E, arylsulfatase-A, AS-A, ASA, cerebroside sulfatase, cerebroside sulfate sulfatase, Cerebroside-sulfatase, sulfatase, cerebroside

ECTree

3.1 Acting on ester bonds

3.1.6 Sulfuric-ester hydrolases

3.1.6.8 cerebroside-sulfatase

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Results	in table
53	Activating Compound
109	AA Sequence
19	Application
1	CAS Registry Number
20	Cloned(Commentary)
4	Crystallization (Commentary)
135	Disease/ Diagnostics
8	General Information
3	General Stability
106	Inhibitors
47	KM Value [mM]
36	Localization
8	Metals/Ions
27	Molecular Weight [Da]
61	Natural Substrates/ Products (Substrates)
86	Organism
2	Pathway
7	PDB ID
26	pH Optimum
4	pH Range
13	Posttranslational Modification
77	Protein Variants
21	Purification (Commentary)
20	Reaction
1	Reaction Type
85	Reference
70	Source Tissue
29	Specific Activity [micromol/min/mg]
15	Storage Stability
176	Substrates and Products (Substrate)
26	Subunits
55	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
4	Temperature Stability [°C]
3	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 3.1.6.8 - cerebroside-sulfatase

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0.000028

-

endogenous activity in HeLa cells

0.00048

-

arylsulfatase fused with GFP expressed in HeLa cells

0.00086

-

before tamoxifen treatment

0.0009

-

3 months after treatment with 40 mg tamoxifen for one week

0.00096

-

arylsulfatase expressed in HeLa cells

0.00127

-

arylsulfatase tagged with hemagglutinin expressed in HeLa cells

0.0013

-

6 months after treatment with 40 mg tamoxifen for one week

0.001393

-

healthy female, comparison of activities at different ages

0.001593

-

female with benign breast disease, comparison of activities at different ages

0.0018

-

in serum

0.00208

-

-

0.002233

-

female with breast cancer, comparison of activities at different ages

0.0039

-

in liver

0.0148

-

arylsulfatase A co-expressed with formylglycine-generating enzyme, in liver

0.0272

-

arylsulfatase A co-expressed with formylglycine-generating enzyme, in serum

1.5

-

nitrocatechol sulfate

135

Oryctolagus cuniculus

-

nitrocatechol sulfate

1393

-

healthy woman, comparison of activities at different ages

1593

-

woman with benign breast disease, comparison of activities at different ages

2233

-

woman with breast cancer, comparison of activities at different ages

24

-

66 kDa form

3.633

Acipenser gueldenstaedtii

-

spermatozoon, 0.5 M acetate buffer, pH 4.9, 37°C

4.316

Acipenser gueldenstaedtii

-

milt plasma, 0.5 M acetate buffer, pH 4.9, 37°C

42

Oryctolagus cuniculus

-

nitrocatechol sulfate

5

-

degraded enzyme

60

-

purified enzyme, 66 kDa form

69.2

-

nitrocatechol sulfate

additional information

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Release 2023.1 (January 2023)