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3.1.6.19: (R)-specific secondary-alkylsulfatase (type III)

This is an abbreviated version!
For detailed information about (R)-specific secondary-alkylsulfatase (type III), go to the full flat file.

Word Map on EC 3.1.6.19

Reaction

an (R)-secondary-alkyl sulfate
+
H2O
=
an (S)-secondary-alcohol
 +
sulfate

Synonyms

(R)-specific sec-alkylsulfatase, Pisa1, S3 secondary alkylsulphohydrolase, sec-alkylsulfatase, sulfatase RS2

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.6 Sulfuric-ester hydrolases
                3.1.6.19 (R)-specific secondary-alkylsulfatase (type III)

Engineering

Engineering on EC 3.1.6.19 - (R)-specific secondary-alkylsulfatase (type III)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F250G
190fold reduction in catalytic activity
H317A
1300fold decrease in kcat, whereas Km is only slightly increased
S233F/F250G
three-fold to four-fold times lower kcat value and a 10fold higer Km value, variant displays activity towardstowards (S)-2-octyl sulfate
S233F/F250S
about 30% of wild-type activity
S233Y
2.5fold reduction in catalytic activity
S233Y/F250G
three-fold to four-fold times lower kcat value
Y417F
114fold decrease in kcat value
Y417H
238 fold decrease in kcat value