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2.5.1.59: protein geranylgeranyltransferase type I

This is an abbreviated version!
For detailed information about protein geranylgeranyltransferase type I, go to the full flat file.

Word Map on EC 2.5.1.59

Reaction

geranylgeranyl diphosphate
+
protein-cysteine
=
S-geranylgeranyl-protein
 +
diphosphate

Synonyms

CAAX geranylgeranyltransferase, CaaX prenyltransferase, CaGGTase-I, CDC43, geranylgeranyl protein transferase type I, geranylgeranyltransferase I, geranylgeranyltransferase type I, geranylgeranyltransferase type-I, geranylgeranyltransferase-1, geranylgeranyltransferase-I, geranylgeranyltransferaseI, GGT, GGT I, GGTalpha, GGTase, GGTase I, Ggtase-1, GGTase-I, GGTaseI, GGTbeta, GGTI, mammalian GGTase-I, PGGT, PGGT beta, PGGT I, PGGT-I, PGGTase-I, PGGTaseI, Ppggb, protein geranylgeranyltransferase, protein geranylgeranyltransferase I, protein geranylgeranyltransferase type I, protein geranylgeranyltransferase type-I, protein geranylgeranyltransferase-I, type I protein geranylgeranyltransferase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.59 protein geranylgeranyltransferase type I

Cloned

Cloned on EC 2.5.1.59 - protein geranylgeranyltransferase type I

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
alpha-subunit encoded by RAM2 and beta-subunit encoded by CDC43 translationally coupled by overlapping the RAM-CDC43 stop-start codons and by locating a ribosome-binding site near the 3' end of RAM2, recombinant enzyme overproduced in Escherichia coli
-
alpha-subunit encoded by RAM2 cloned to the pFC vector, which has an chloroamphenicol resistance gene and beta-subunit encoded by CAL1 cloned to the pFlag vector, which has an ampicillin resistance gene, these two recombinant enzymes are cotransformed and expressed in Escherichia coli
-
coexpression of two subunits of enzyme by Sf9 cells infected with recombinant baculovirus
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 (DE3) and Sf9 insect cells
-
expressed in Escherichia coli strain BL21(DE3) GGPT/pET23a
-
expressed in Sf9 insect cells
gene cdc43, encoding the Ggtase-I beta-subunit, DNA and amino acid sequence determination and analysis, recombinant expression of wild-ype and mutant enzymes
identification of the cwp1+ gene, which encodes the alpha-subunit of enzyme, coexpression of cwp1p and cwg2p, beta-subunit, in Escherichia coli
-
PCR-amplification and expression in Escherichia coli
PCR-amplification of human enzyme for RT-PCR analysis of expression in human trabecular mesh cells
-
recombinant expression of GST-tagged GGTIbeta subunit
recombinant mammalian enzyme expressed in Sf9 cells
-
wild-type and mutant enzymes expressed in Escherichia coli
-