1.4.99.6: D-arginine dehydrogenase
This is an abbreviated version!
For detailed information about D-arginine dehydrogenase, go to the full flat file.
Word Map on EC 1.4.99.6
Reaction
Synonyms
D-alanine dehydrogenase, D-amino acid dehydrogenase, D-amino acid dehydrogenase DadA, DAADH, DAD, DadA, DADH, DauA, EC 1.4.99.1, NADP+-dependent D-amino acid dehydrogenase
ECTree
1.4.99.6 D-arginine dehydrogenaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 1.4.99.6 - D-arginine dehydrogenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
D-alanine + H2O + 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
D-alanine + H2O + methylene blue
pyruvate + NH3 + reduced methylene blue
-
low activity
-
?
D-arginine + H2O + FAD
5-guanidino-2-oxopentanoate + NH3 + FADH2
the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity
-
-
?
D-arginine + H2O + iodonitrotetrazolium chloride
5-guanidino-2-oxopentanoate + NH3 + reduced iodonitrotetrazolium chloride
-
D-arginine and D-lysine are the most effective substrates
-
-
?
D-arginine + phenazine methosulfate
5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
D-arginine + phenazine methosulfate + H2O
5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
D-Asp + H2O + oxidized 2,6-dichlorophenolindophenol
2-oxobutanedioate + NH3 + reduced 2,6-dichlorophenolindophenol
D-asparagine + H2O + 2,6-dichlorophenolindophenol
2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-asparagine + H2O + methylene blue
2-oxosuccinamic acid + NH3 + reduced methylene blue
-
-
-
?
D-asparagine + phenazine methosulfate + H2O
2-oxobutanedioate + NH3 + reduced phenazine methosulfate
D-cysteine + H2O + methylene blue
3-mercapto-2-oxopropanoate + NH3 + reduced methylene blue
-
-
-
?
D-cystine + H2O + methylene blue
? + NH3 + reduced methylene blue
-
-
-
?
D-Glu + H2O + oxidized 2,6-dichlorophenolindophenol
2-oxoglutarate + NH3 + reduced 2,6-dichlorophenolindophenol
D-glutamine + phenazine methosulfate + H2O
5-amino-2,5-dioxopentanoate + NH3 + reduced phenazine methosulfate
D-histidine + H2O + 2,6-dichlorophenolindophenol
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-histidine + H2O + FAD
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + FADH2
the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity
-
-
?
D-histidine + H2O + iodonitrotetrazolium chloride
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced iodonitrotetrazolium chloride
-
-
-
-
?
D-histidine + H2O + methylene blue
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced methylene blue
-
-
-
?
D-histidine + phenazine methosulfate
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced phenazine methosulfate
D-histidine + phenazine methosulfate + H2O
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-isoleucine + H2O + 2,6-dichlorophenolindophenol
3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-isoleucine + H2O + methylene blue
3-methyl-2-oxopentanoate + NH3 + reduced methylene blue
-
-
-
?
D-isoleucine + phenazine methosulfate + H2O
3-methyl-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-kynurenine + H2O + 2,6-dichlorophenolindophenol
4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-kynurenine + H2O + methylene blue
4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced methylene blue
-
-
-
?
D-leucine + H2O + 2,6-dichlorophenolindophenol
4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-leucine + H2O + methylene blue
4-methyl-2-oxopentanoate + NH3 + reduced methylene blue
-
-
-
?
D-leucine + phenazine methosulfate + H2O
4-methyl-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-lysine + H2O + iodonitrotetrazolium chloride
7-amino-2-oxoheptanoic acid + NH3 + reduced iodonitrotetrazolium chloride
-
D-arginine and D-lysine are the most effective substrates
-
-
?
D-lysine + phenazine methosulfate
6-amino-2-oxohexanoate + NH3 + reduced phenazine methosulfate
D-lysine + phenazine methosulfate + H2O
7-amino-2-oxoheptanoic acid + NH3 + reduced phenazine methosulfate
-
-
-
?
D-Met + H2O + oxidized 2,6-dichlorophenolindophenol
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
D-methionine + H2O + 2,6-dichlorophenolindophenol
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-methionine + H2O + iodonitrotetrazolium chloride
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced iodonitrotetrazolium chloride
-
-
-
-
?
D-methionine + phenazine methosulfate
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced phenazine methosulfate
D-methionine + phenazine methosulfate + H2O
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-norleucine + H2O + 2,6-dichlorophenolindophenol
2-oxohexanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-norleucine + H2O + methylene blue
2-oxohexanoate + NH3 + reduced methylene blue
-
-
-
?
D-norvaline + H2O + methylene blue
2-oxopentanoate + NH3 + reduced methylene blue
-
-
-
?
D-ornithine + H2O + iodonitrotetrazolium chloride
? + NH3 + reduced iodonitrotetrazolium chloride
-
-
-
-
?
D-Phe + H2O + oxidized 2,6-dichlorophenolindophenol
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
19.8% activity compared to D-Pro
-
-
?
D-phenylalanine + H2O + 2,6-dichlorophenolindophenol
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
D-phenylalanine + H2O + FAD
phenylpyruvate + NH3 + FADH2
the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity
-
-
?
D-phenylalanine + H2O + iodonitrotetrazolium chloride
phenylpyruvate + NH3 + reduced iodonitrotetrazolium chloride
-
-
-
-
?
D-phenylalanine + H2O + methylene blue
phenylpyruvate + NH3 + reduced methylene blue
-
-
-
?
D-phenylalanine + phenazine methosulfate
2-oxo-3-phenylpropanoate + NH3 + reduced phenazine methosulfate
-
overall reaction
-
?
D-phenylalanine + phenazine methosulfate + H2O
2-oxo-3-phenylpropanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-Pro + H2O + oxidized 2,6-dichlorophenolindophenol
DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
100% activity
-
-
?
D-proline + H2O + 2,6-dichlorophenolindophenol
DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-proline + H2O + coenzyme Q1
DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1
-
-
-
?
D-proline + H2O + FAD
2-oxopentanoic acid + NH3 + FADH2
the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity
-
-
?
D-proline + phenazine methosulfate + H2O
DELTA1-pyrroline-2-carboxylate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-Ser + H2O + oxidized 2,6-dichlorophenolindophenol
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
6.7% activity compared to D-Pro
-
-
?
D-serine + H2O + 2,6-dichlorophenolindophenol
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
D-serine + H2O + FAD
3-hydroxy-2-oxopropanoate + NH3 + FADH2
the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity
-
-
?
D-serine + H2O + methylene blue
3-hydroxy-2-oxopropanoate + NH3 + reduced methylene blue
-
low activity
-
?
D-serine + phenazine methosulfate + H2O
3-hydroxy-2-oxopropanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-threonine + H2O + 2,6-dichlorophenolindophenol
3-hydroxy-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
low activity
-
?
D-threonine + H2O + FAD
3-hydroxy-2-oxobutanoate + NH3 + FADH2
the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity
-
-
?
D-threonine + H2O + methylene blue
3-hydroxy-2-oxobutanoate + NH3 + reduced methylene blue
-
low activity
-
?
D-threonine + phenazine methosulfate + H2O
3-hydroxy-2-oxobutanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-tryptophan + H2O + 2,6-dichlorophenolindophenol
3-indole-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-tryptophan + H2O + methylene blue
3-indole-2-oxopropanoate + NH3 + reduced methylene blue
-
-
-
?
D-tryptophan + phenazine methosulfate + H2O
3-indole-2-oxopropanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-tyrosine + H2O + 2,6-dichlorophenolindophenol
3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-tyrosine + H2O + FAD
(4-hydroxyphenyl)pyruvate + NH3 + FADH2
the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity
-
-
?
D-tyrosine + H2O + iodonitrotetrazolium chloride
3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced iodonitrotetrazolium chloride
-
-
-
-
?
D-tyrosine + H2O + methylene blue
3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced methylene blue
-
-
-
?
D-tyrosine + phenazine methosulfate
3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced phenazine methosulfate
-
overall reaction
-
?
D-tyrosine + phenazine methosulfate + H2O
(4-hydroxyphenyl)pyruvate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-Val + H2O + oxidized 2,6-dichlorophenolindophenol
2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
0.8% activity compared to D-Pro
-
-
?
D-valine + H2O + 2,6-dichlorophenolindophenol
2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-valine + H2O + FAD
2-oxopentanoate + NH3 + FADH2
the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity
-
-
?
D-valine + H2O + methylene blue
2-oxoisopentanoate + NH3 + reduced methylene blue
-
-
-
?
D-valine + phenazine methosulfate + H2O
2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
L-Ala + H2O + oxidized 2,6-dichlorophenolindophenol
?
1.9% activity compared to D-Pro
-
-
?
L-Pro + H2O + oxidized 2,6-dichlorophenolindophenol
?
3.5% activity compared to D-Pro
-
-
?
(3S,3R)-2-oxo-3-methylvaleric acid + NH3 + NADPH + H+
D-isoleucine + H2O + NADP+
-
-
yield of 45% after incubation for 1 h at 50 °C
-
?
(3S,3R)-2-oxo-3-methylvaleric acid + NH3 + NADPH + H+
D-isoleucine + H2O + NADP+
-
-
yield of 45% after incubation for 1 h at 50 °C
-
?
2-oxo-3-methylbutyric acid + NH3 + NADPH + H+
D-valine + H2O + NADP+
-
-
yield of 83% after incubation for 1 h at 50 °C
-
?
2-oxo-3-methylbutyric acid + NH3 + NADPH + H+
D-valine + H2O + NADP+
-
-
yield of 83% after incubation for 1 h at 50 °C
-
?
2-oxo-4-methylvaleric acid + NH3 + NADPH + H+
D-leucine + H2O + NADP+
-
-
yield of 91% after incubation for 1 h at 50 °C
-
?
2-oxo-4-methylvaleric acid + NH3 + NADPH + H+
D-leucine + H2O + NADP+
-
-
yield of 91% after incubation for 1 h at 50 °C
-
?
D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
36.8% activity compared to D-Pro
-
-
?
D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
36.8% activity compared to D-Pro
-
-
?
D-alanine + H2O + 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-alanine + H2O + 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
low activity
-
?
D-alanine + H2O + FAD
pyruvate + NH3 + FADH2
D-amino acid dehydrogenase activity of broad substrate specificity
-
-
?
D-alanine + H2O + FAD
pyruvate + NH3 + FADH2
the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity
-
-
?
D-alanine + phenazine methosulfate + H2O
pyruvate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-alanine + phenazine methosulfate + H2O
pyruvate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-arginine + phenazine methosulfate
5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
overall reaction
-
?
D-arginine + phenazine methosulfate
5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
-
overall reaction
-
?
D-arginine + phenazine methosulfate
5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
overall reaction
-
?
D-arginine + phenazine methosulfate
5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
fast substrate D-arginine leads to a dead-end complex of the reduced enzyme and the substrate at high concentrations of D-arginine yielding substrate inhibition, while the overall turnover is partially limited by the release of the iminoarginine product
overall reaction
-
?
D-arginine + phenazine methosulfate + H2O
5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-arginine + phenazine methosulfate + H2O
5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
overall reaction
-
?
D-arginine + phenazine methosulfate + H2O
5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
overall reaction
-
?
D-arginine + phenazine methosulfate + H2O
5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-Asp + H2O + oxidized 2,6-dichlorophenolindophenol
2-oxobutanedioate + NH3 + reduced 2,6-dichlorophenolindophenol
0.8% activity compared to D-Pro
-
-
?
D-Asp + H2O + oxidized 2,6-dichlorophenolindophenol
2-oxobutanedioate + NH3 + reduced 2,6-dichlorophenolindophenol
0.8% activity compared to D-Pro
-
-
?
D-asparagine + phenazine methosulfate + H2O
2-oxobutanedioate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-asparagine + phenazine methosulfate + H2O
2-oxobutanedioate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-Glu + H2O + oxidized 2,6-dichlorophenolindophenol
2-oxoglutarate + NH3 + reduced 2,6-dichlorophenolindophenol
1.0% activity compared to D-Pro
-
-
?
D-Glu + H2O + oxidized 2,6-dichlorophenolindophenol
2-oxoglutarate + NH3 + reduced 2,6-dichlorophenolindophenol
1.0% activity compared to D-Pro
-
-
?
D-glutamine + phenazine methosulfate + H2O
5-amino-2,5-dioxopentanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-glutamine + phenazine methosulfate + H2O
5-amino-2,5-dioxopentanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-histidine + phenazine methosulfate
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced phenazine methosulfate
-
overall reaction
-
?
D-histidine + phenazine methosulfate
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced phenazine methosulfate
slow substrate, the initial Michaelis complex undergoes an isomerization involving multiple conformations that are not all equally catalytically competent for the subsequent oxidation reaction, while the overall turnover is at least partially limited by flavin reduction
-
-
?
D-leucine + phenazine methosulfate
? + NH3 + reduced phenazine methosulfate
-
-
-
?
D-leucine + phenazine methosulfate
? + NH3 + reduced phenazine methosulfate
-
overall reaction
-
?
D-leucine + phenazine methosulfate
? + NH3 + reduced phenazine methosulfate
enzyme preferentially binds the zwitterionic form of the substrate. Isomerization of the Michaelis complex yields an enzyme-substrate complex competent for flavin reduction. Amine deprotonation triggers the oxidation reaction, with cleavage of the substrate NH and CH bonds occurring in an asynchronous fashion. Tyr53, on a mobile loop covering the active site, may participate in substrate binding and facilitate flavin reduction
-
-
?
D-lysine + phenazine methosulfate
6-amino-2-oxohexanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-lysine + phenazine methosulfate
6-amino-2-oxohexanoate + NH3 + reduced phenazine methosulfate
-
overall reaction
-
?
D-Met + H2O + oxidized 2,6-dichlorophenolindophenol
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
0.8% activity compared to D-Pro
-
-
?
D-Met + H2O + oxidized 2,6-dichlorophenolindophenol
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
0.8% activity compared to D-Pro
-
-
?
D-methionine + phenazine methosulfate
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced phenazine methosulfate
-
-
-
?
D-methionine + phenazine methosulfate
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced phenazine methosulfate
-
overall reaction
-
?
D-phenylalanine + H2O + 2,6-dichlorophenolindophenol
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-phenylalanine + H2O + 2,6-dichlorophenolindophenol
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-Pro + H2O + coenzyme Q1
?
D-Pro is the best substrate
-
-
?
D-serine + H2O + 2,6-dichlorophenolindophenol
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-serine + H2O + 2,6-dichlorophenolindophenol
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
low activity
-
?
additional information
?
-
no substrates: aspartae, glutamate
-
-
-
additional information
?
-
no substrates: aspartae, glutamate
-
-
-
