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Literature summary for 1.4.99.6 extracted from

  • Tsukada, K.
    D-Amino acid dehydrogenase (Pseudomonas fluorescens) (1971), Methods Enzymol., 17B, 623-624.
No PubMed abstract available

General Stability

General Stability Organism
very unstable Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
2,6-dichlorophenolindophenol inhibition of the dichlorophenolindophenol-specific enzyme at high concentrations Pseudomonas fluorescens
D-Amino acids various D-amino acids competitively inhibit D-kynurenine deamination Pseudomonas fluorescens
p-chloromercuribenzoic acid 90% inhibition of the methylene blue specific, 70% inhibition of the dichlorophenolindophenol specific enzyme at 0.5 mM Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
methylene blue specific acceptor, cosubstrate: D-kynurenine Pseudomonas fluorescens
0.07
-
2,6-dichlorophenolindophenol specific acceptor, cosubstrate: D-kynurenine Pseudomonas fluorescens
0.45
-
D-kynurenine both methylene blue and 2,6-dichlorophenolindophenol specific enzymes Pseudomonas fluorescens
0.47
-
D-tryptophan both methylene blue and 2,6-dichlorophenolindophenol specific enzymes Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity, two enzymes with different acceptor specificities, fractionation and chromatography techniques Pseudomonas fluorescens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture one methylene blue specific, one 2,6-dichlorophenolindophenol specific enzyme, the methylene blue specific enzyme is only detectable in D-tryptophan grown cells Pseudomonas fluorescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.113
-
methylene blue specific enzyme Pseudomonas fluorescens
0.213
-
2,6-dichlorophenolindophenol specific enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + 2,6-dichlorophenolindophenol low activity Pseudomonas fluorescens pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-alanine + H2O + methylene blue low activity Pseudomonas fluorescens pyruvate + NH3 + reduced methylene blue
-
?
D-asparagine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-asparagine + H2O + methylene blue
-
Pseudomonas fluorescens 2-oxosuccinamic acid + NH3 + reduced methylene blue
-
?
D-cysteine + H2O + methylene blue
-
Pseudomonas fluorescens 3-mercapto-2-oxopropanoate + NH3 + reduced methylene blue
-
?
D-cystine + H2O + methylene blue
-
Pseudomonas fluorescens ? + NH3 + reduced methylene blue
-
?
D-histidine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-histidine + H2O + methylene blue
-
Pseudomonas fluorescens 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced methylene blue
-
?
D-isoleucine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-isoleucine + H2O + methylene blue
-
Pseudomonas fluorescens 3-methyl-2-oxopentanoate + NH3 + reduced methylene blue
-
?
D-kynurenine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-kynurenine + H2O + methylene blue
-
Pseudomonas fluorescens 4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced methylene blue
-
?
D-leucine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-leucine + H2O + methylene blue
-
Pseudomonas fluorescens 4-methyl-2-oxopentanoate + NH3 + reduced methylene blue
-
?
D-methionine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-norleucine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 2-oxohexanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-norleucine + H2O + methylene blue
-
Pseudomonas fluorescens 2-oxohexanoate + NH3 + reduced methylene blue
-
?
D-norvaline + H2O + methylene blue
-
Pseudomonas fluorescens 2-oxopentanoate + NH3 + reduced methylene blue
-
?
D-phenylalanine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-phenylalanine + H2O + methylene blue
-
Pseudomonas fluorescens phenylpyruvate + NH3 + reduced methylene blue
-
?
D-serine + H2O + 2,6-dichlorophenolindophenol low activity Pseudomonas fluorescens 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-serine + H2O + methylene blue low activity Pseudomonas fluorescens 3-hydroxy-2-oxopropanoate + NH3 + reduced methylene blue
-
?
D-threonine + H2O + 2,6-dichlorophenolindophenol low activity Pseudomonas fluorescens 3-hydroxy-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-threonine + H2O + methylene blue low activity Pseudomonas fluorescens 3-hydroxy-2-oxobutanoate + NH3 + reduced methylene blue
-
?
D-tryptophan + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 3-indole-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-tryptophan + H2O + methylene blue
-
Pseudomonas fluorescens 3-indole-2-oxopropanoate + NH3 + reduced methylene blue
-
?
D-tyrosine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-tyrosine + H2O + methylene blue
-
Pseudomonas fluorescens 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced methylene blue
-
?
D-valine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
D-valine + H2O + methylene blue
-
Pseudomonas fluorescens 2-oxoisopentanoate + NH3 + reduced methylene blue
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 both methylene blue and 2,6-dichlorophenolindophenol specific enzymes Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas fluorescens