Crystallization (Comment) | Organism |
---|---|
structure without added D-amino acids, to 1.06 A resolution, and in presence of D-arginine and D-histidine | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
D-arginine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.26 | - |
D-Lysine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.8 | - |
D-tyrosine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
10 | - |
D-methionine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
11 | - |
D-phenylalanine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
11 | - |
D-histidine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
12 | - |
D-leucine | pH 8.7, 25°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HXE3 | - |
- |
Pseudomonas aeruginosa DSM 22644 | Q9HXE3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | pyruvate + NH3 + reduced phenazine methosulfate | - |
? | |
D-alanine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa DSM 22644 | pyruvate + NH3 + reduced phenazine methosulfate | - |
? | |
D-arginine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-arginine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa DSM 22644 | 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-asparagine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 2-oxobutanedioate + NH3 + reduced phenazine methosulfate | - |
? | |
D-asparagine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa DSM 22644 | 2-oxobutanedioate + NH3 + reduced phenazine methosulfate | - |
? | |
D-glutamine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 5-amino-2,5-dioxopentanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-glutamine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa DSM 22644 | 5-amino-2,5-dioxopentanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-histidine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-isoleucine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 3-methyl-2-oxopentanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-leucine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 4-methyl-2-oxopentanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-lysine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 7-amino-2-oxoheptanoic acid + NH3 + reduced phenazine methosulfate | - |
? | |
D-methionine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-phenylalanine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 2-oxo-3-phenylpropanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-proline + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | DELTA1-pyrroline-2-carboxylate + NH3 + reduced phenazine methosulfate | - |
? | |
D-serine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 3-hydroxy-2-oxopropanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-threonine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 3-hydroxy-2-oxobutanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-tryptophan + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 3-indole-2-oxopropanoate + NH3 + reduced phenazine methosulfate | - |
? | |
D-tyrosine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | (4-hydroxyphenyl)pyruvate + NH3 + reduced phenazine methosulfate | - |
? | |
D-valine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 2-oxopentanoate + NH3 + reduced phenazine methosulfate | - |
? | |
additional information | no substrates: aspartae, glutamate | Pseudomonas aeruginosa | ? | - |
- |
|
additional information | no substrates: aspartae, glutamate | Pseudomonas aeruginosa DSM 22644 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
DADH | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.4 | - |
D-leucine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
23 | - |
D-tyrosine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
35 | - |
D-histidine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
75 | - |
D-phenylalanine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
141 | - |
D-Lysine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
154 | - |
D-methionine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
204 | - |
D-arginine | pH 8.7, 25°C | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains a noncovalently linked FAD | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
metabolism | DADH catalyzes an asynchronous CH and NH bond cleavage via a hydride transfer mechanism. The enzyme follows a ping-pong bibi mechanism. The shape and flexibility of loop L1 in the active site of DADH are important for substrate capture and broad substrate specificity | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00075 | - |
D-threonine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.0038 | - |
D-serine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.016 | - |
D-asparagine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.041 | - |
D-alanine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.047 | - |
D-valine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.186 | - |
D-glutamine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.195 | - |
D-isoleucine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.245 | - |
D-tryptophan | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.42 | - |
D-proline | pH 8.7, 25°C | Pseudomonas aeruginosa | |
0.515 | - |
D-leucine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
3.14 | - |
D-histidine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
6.9 | - |
D-phenylalanine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
14.8 | - |
D-methionine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
27.6 | - |
D-tyrosine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
530 | - |
D-Lysine | pH 8.7, 25°C | Pseudomonas aeruginosa | |
3400 | - |
D-arginine | pH 8.7, 25°C | Pseudomonas aeruginosa |