1.20.4.1: arsenate reductase (glutathione/glutaredoxin)
This is an abbreviated version!
For detailed information about arsenate reductase (glutathione/glutaredoxin), go to the full flat file.
Word Map on EC 1.20.4.1
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1.20.4.1
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arsenite
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asiii
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arsr
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glutaredoxins
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arsenic-resistant
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pteris
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hyperaccumulating
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metalloid
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phytochelatins
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vittata
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as-contaminated
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cdc25
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pentavalent
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arsenate-reducing
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as-resistant
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frond
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arsenic-rich
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arsenate-sensitive
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degradation
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antimonite
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arsenic-contaminated
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monomethylarsonic
- 1.20.4.1
- arsenite
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asiii
- arsr
- glutaredoxins
-
arsenic-resistant
- pteris
-
hyperaccumulating
-
metalloid
- phytochelatins
- vittata
-
as-contaminated
- cdc25
-
pentavalent
-
arsenate-reducing
-
as-resistant
- frond
-
arsenic-rich
-
arsenate-sensitive
- degradation
- antimonite
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arsenic-contaminated
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monomethylarsonic
Reaction
Synonyms
Acr2, Acr2p, ACR3-ArsC, All0195, AR, ARSA, ArsC, arsenate reductase, arsenate reductase (glutaredoxin), AsV reductase, EC 1.97.1.5, gene arsC proteins, glutaredoxin, glutathione:arsenate oxidoreductase, Grx, MXAN_0575, PcAcr2, proteins (specific proteins and subclasses), gene arsC, PvGRX5, reductase, arsenate, slr0946, SynArsC
ECTree
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Substrates Products
Substrates Products on EC 1.20.4.1 - arsenate reductase (glutathione/glutaredoxin)
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REACTION DIAGRAM
arsenate + glutaredoxin 1
arsenite + glutaredoxin 1 disulfide + H2O
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-
-
-
r
arsenate + glutaredoxin 2
arsenite + glutaredoxin 1 disulfide + H2O
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-
-
-
r
arsenate + glutaredoxin 2
arsenite + glutaredoxin 2 disulfide + H2O
-
-
-
-
r
arsenate + glutaredoxin 3
arsenite + glutaredoxin 1 disulfide + H2O
-
-
-
-
r
arsenate + glutaredoxin 3
arsenite + glutaredoxin 3 disulfide + H2O
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-
-
-
r
arsenate + reduced glutathione + NAD+ + glyceraldehyde-3-phosphate
arsenite + glutathione + ?
arsenate + glutaredoxin
arsenite + glutaredoxin disulfide + H2O
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-
-
-
?
arsenate + glutaredoxin
arsenite + glutaredoxin disulfide + H2O
-
-
-
-
?
arsenate + glutaredoxin
arsenite + glutaredoxin disulfide + H2O
-
-
-
-
?
arsenite + glutaredoxin C7 disulfide + H2O
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-
-
-
?
arsenate + glutaredoxin C7
arsenite + glutaredoxin C7 disulfide + H2O
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-
-
-
?
arsenite + glutaredoxin C72.1 disulfide + H2O
-
-
-
-
?
arsenate + glutaredoxin C72.1
arsenite + glutaredoxin C72.1 disulfide + H2O
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-
-
-
?
arsenite + oxidized glutaredoxin
glutaredoxin functions as the electron donor for arsenate reduction
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-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
glutaredoxin functions as the electron donor for arsenate reduction
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-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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strong specificity for arsenate
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-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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the first step of the reaction is the binding of arsenate, followed by the interaction of the enzyme-arsenate complex with GSH. A reaction scheme is hypothesized in which the enzyme forms a mixed disulfide between the Cys-12 thiolate of ArsC and GSH. Glutaredoxin would then be required to resolve the mixed disulfide, regenerating reduced ArsC
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-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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thioredoxin is not effective as electron donor
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-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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the enzyme uses GSH with glutaredoxin as electron donor. Glutaredoxin 2 is the most effective hydrogen donor for the reduction of arsenate. During the catalytic cycle, ArsC forms a mixed disulfide with GSH before being reduced by glutaredoxin to regenerate the active ArsC reductase
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-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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C12 is located at the active site and is required for catalysis
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-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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the enzyme is involved in bacterial arsenic resistance
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?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
-
the enzyme is involved in bacterial arsenic resistance
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?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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the enzyme is involved in bacterial arsenic resistance
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?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
-
the enzyme is involved in bacterial arsenic resistance
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?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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the enzyme is involved in bacterial arsenic resistance
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?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
enzyme catalyzes the oxidation of NADPH coupled with reduction of arsenate in the presence of glutathione reductase, glutathione and glutaredoxin
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-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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-
-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
enzyme catalyzes the oxidation of NADPH coupled with reduction of arsenate in the presence of glutathione reductase, glutathione and glutaredoxin
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-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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-
-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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thioredoxin is unable to support arsenate reduction. The N-terminal Cys residue is essential for arsenate reductase activity. During the catalytic cycle, Acr2p forms a mixed disulfide with GSH before being reduced vby glutaredoxin to regenerate the active Acr2p reductase
-
?
arsenate + reduced glutaredoxin
arsenite + oxidized glutaredoxin
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-
-
?
arsenate + reduced glutathione
arsenite + glutathione
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enzyme plays an important role in detoxification of arsenate
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-
?
arsenite + glutathione + ?
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-
-
-
?
arsenate + reduced glutathione + NAD+ + glyceraldehyde-3-phosphate
arsenite + glutathione + ?
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-
-
-
?
?
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-
the enzyme exhibits weak phosphatase activity toward 4-nitrophenyl phosphate
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-
?
additional information
?
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both glutathione-SH and glutaredoxin are required for activity. No substrate: phosphate
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-
?
additional information
?
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both glutathione-SH and glutaredoxin are required for activity. No substrate: phosphate
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-
?
additional information
?
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-
chemical shift assignments of 1H, 13C and 15N atoms for the reduced form the enzyme
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-
?
additional information
?
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chemical shift assignments of 1H, 13C and 15N atoms for the reduced form the enzyme
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-
?
additional information
?
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-
the enzyme requires the glutaredoxin system for its reactivation
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-
?