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1.20.4.1: arsenate reductase (glutathione/glutaredoxin)

This is an abbreviated version!
For detailed information about arsenate reductase (glutathione/glutaredoxin), go to the full flat file.

Word Map on EC 1.20.4.1

Reaction

arsenate
+
glutathione
 +
glutaredoxin
=
arsenite
 +
a glutaredoxin-glutathione disulfide
 +
H2O

Synonyms

Acr2, Acr2p, ACR3-ArsC, All0195, AR, ARSA, ArsC, arsenate reductase, arsenate reductase (glutaredoxin), AsV reductase, EC 1.97.1.5, gene arsC proteins, glutaredoxin, glutathione:arsenate oxidoreductase, Grx, MXAN_0575, PcAcr2, proteins (specific proteins and subclasses), gene arsC, PvGRX5, reductase, arsenate, slr0946, SynArsC

ECTree

     1 Oxidoreductases
         1.20 Acting on phosphorus or arsenic in donors
             1.20.4 With disulfide as acceptor
                1.20.4.1 arsenate reductase (glutathione/glutaredoxin)

Cloned

Cloned on EC 1.20.4.1 - arsenate reductase (glutathione/glutaredoxin)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the bacterial expression vector and expressed in Escherichia coli as a C-terminal histidine-tagged protein
-
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli
expression in Escherichia coli complements phenotypes
-
gene Pv5-6 encoding PvGrx5 expressed in Escherichia coli strain XL-1 Blue. PvGrx5 expressed in Escherichia coli mutants in which the arsenic resistance genes of the ars operon are deleted (strain AW3110), a deletion of the gene for the ArsC arsenate reductase (strain WC3110), and a strain in which the ars operon is deleted and the gene for the GlpF aquaglyceroporin is disrupted (strain OSBR1)
sequence contains a variation of conserved motif HCX5R where R is replaced by S